摘要
目的:通过观察杭白芷香豆素(CAD)对戊巴比妥钠和巴比妥钠催眠作用的影响,初步探讨其中枢作用及其发生机制。方法:将小鼠随机分为溶媒对照组(5%吐温-80;20 ml/kg)、氯霉素阳性对照组(25 mg/kg)和CAD组(25,50,100 mg/kg)。等容量灌胃给药1 h后,再腹腔注射戊巴比妥钠40 mg/kg或巴比妥钠200 mg/kg,观察小鼠催眠潜伏期和睡眠时间。结果:氯霉素组和3个剂量的CAD组均可使戊巴比妥钠睡眠时间延长,分别延至溶媒对照组的380%、284%、353%和366%(P<0.01);100 mg/kg CAD组还可显著延长巴比妥钠催眠潜伏期(P<0.01),并缩短其睡眠时间(P<0.01)。结论:CAD具有一定中枢兴奋作用,但同时可能具有肝药酶抑制作用。
Objective： To survey the influence and mechanism of the coumarins from Radix Angelicae Dahuricae （CAD） on the hypnotic effects of pentobarbital sodium and barbital sodium in mice. Methods： Sixty Kunming mice were randomly divided into five groups, with 12 animals in each group. Mice in group 1, 2, 3, 4 and 5 were given each with 20 ml/kg gastrogavage （5% tween80）, 25 mg/kg of chloromycin, and 25,50,100 mg/kg of CAD in equal volumes, respectively. One hour later, all of the animals were given＇each an intraperitoneal injection of 40 mg/kg of pentobarbital sodium or 200 mg/kg of barbital sodium. The latent period and duration of hypnosis of the mice were recorded under the observation. Results： The duration of hypnosis induced by pentobarbital sodium was prolonged by 380%, 284%, 353% and 366% （P〈0.01）, respectively, in mice given chloromycin and three different doses of CAD. 100 mg/kg of CAD was shown to significantly prolonged the latent period （P〈0.01） and shortened the duration of hypnosis （P〈 0.01） induced by barbital sodium. Conclusion： CAD could inhibit the liver microsomal cytochrome P450 activity. CAD in the higher dose seems to excite the central nervous system in mice.
出处
《武汉大学学报（医学版）》
CAS
2006年第1期63-65,共3页
Medical Journal of Wuhan University
关键词
杭白芷香豆素
戊巴比妥钠
巴比妥钠
催眠作用
Coumarin, Radix Angelicae Dahuricae
Pentobarbital Sodium
Barbital Sodium
Hypnotic Effect
