摘要
目的观察脑囊虫病患者脑脊液中一氧化氮(NO)和肿瘤坏死因子-α(TNF-α)的表达。探讨两者在囊尾蚴感染引起的人体免疫反应中的作用。方法用比色法和双抗夹心ELISA检测了28例脑囊虫病患者实验组和24例对照组脑脊液中一氧化氮(NO)和肿瘤坏死因-α(TNF-α)的表达。结果脑囊虫病患者急性期脑脊液中TNF-α的含量(0.69±0.11)×10-3ng/L,显示高于正常对照组(0.24±0.09)×10-3ng/L(P<0.01);NO的含量(67.77±5.57)umol/L,显著高于正常对照组(38.04±4.13)umol/L(P<0.01)。结论NO与TNF-α参与人体对囊虫的免疫过程,并可能通过某些免疫炎症反应与细胞毒性等作用影响中枢神经正常功能。
To observe the concentration of nitric oxide（NO） and TNF-α in cerebrospinal fluid of patient with cerebral cysticercosis, the levels of TNF-α and NO in CSF of 28 patients with neurocysticercosis and 24 healthy people as control were detected by sandwich-ELISA and colorimerty. The level of TNF-α in patients and normal individuals was （0.69 ± 0.11） × 10^-3 ng/L and （0.24 ± 0.09） × 10^-3 ng/L（ P 〈 0.01 ） ;while that of NO in patients and normal indivivuals were（67.77 ± 5.57）μmol/L and （38.04± 4. 13）μmol/L respectively（ P 〈 0.01 ）. It is concluded that the immune response of patients with cerebral cysticercosis are modulated by TNF-α and NO which could generate inflammatory response and cytoxicity on central nervous system of patients.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2006年第1期83-84,共2页
Chinese Journal of Zoonoses
关键词
脑囊虫
一氧化氧
肿瘤坏死因子
脑脊液
neurocysticercosis
nitric oxide
TNF- α
cerebrospinal fluid
