摘要
目的对四环素预防激素增强Shwartzman反应骨坏死的实验效果进行初步观察。方法将新西兰白兔(35只)随机分为3组。A组:四环素预防组(n=10),于建立骨坏死模型前2d给予四环素,持续10d;B组:骨坏死模型组(n=20);C组:空白对照组(n=5)。于建模第5周、第9周分别给予钙黄绿素、土霉素进行标记。观察动物存活率,9周后获取肱、股骨头标本,计算标本缺血坏死率;脱钙标本行HE染色、Hoechst染色观察细胞凋亡;未脱钙标本行荧光染色观察骨内微循环并计算平均矿化沉积率。结果A、B组动物存活率(分别为80%,25%)、标本缺血坏死率(分别为18.75%,80%)有显著差异(P<0.01);A组核溶解骨细胞数、空骨陷窝数、凋亡指数明显低于B组(P<0.01),略高于C组(P>0.05);A组矿化沉积率[(0.77±0.20)μm·d-1]明显优于B组[(0.54±0.12)μm·d-1]、C组[(0.37±0.07)μm·d-1](P<0.05)。结论四环素能够改善骨内微循环,减少骨细胞凋亡数,防止激素增强的Shwartzman反应骨坏死的发生。
Objective To investigate the effects of tetracycline on steroid-enhanced osteonecrosis induced by shwartzman reaction in rabbit model. Methods New Zealand rabbits （ n = 35） were randomized into three groups. Tetracycline was given 2 days before the animal model replicated in tetracycline-pretreated group （group A, n = 10） ; By modified methods to replicate steroid enhanced osteonecrosis animal model with Shwartzman reaction （group B, n = 20） ; and saline was given in group C as control （ n = 5）. Vital fluorescent bone stains were given at the 5th week （calcein green） or the 9th week（oxytetracycline） postoperatively. The humerus and femur heads were obtained 9 weeks later. The survival rate and incidence of osteonecrosis of these animals were compared among groups. The decalcified specimens were sectioned and stained with hematoxylin and eosin （HE） or Hoechst dye; the microcirculatory obstruction in subchondral area and the mean rate of mineral apposition （MRMA） were also explored in undecalcified specimens. Results The survival rate and ischemic necrosis rate have significant difference between group A（ 80 %, 18.75 % ） and group B （ 25 %, 80 % ） （ P 〈 0.01 ）. The number of empty lacunae, karyolysis, pyknotic nuclei of osteocytes and apoptotic index of group A were lower than those of group B（ P 〈 0.01） and a little higher than those of group C（P 〉 0.05）. The MRMA of group A[（0.77 ± 0.20）μm·d^-1] was higher than that of group B[（0.54± 0.12）μm· d^-1] and group C[（0.37 ± 0.07）μm· d^-1] （P 〈 0.05）. Conclusion Pretreatment with tetracycline could improve microcireulation and decrease osteocytes apoptosis, which might be a new strategy for the prevention and treatment of steroid-induced osteonecrosis.
出处
《国际内分泌代谢杂志》
2006年第1期60-62,i0001,共4页
International Journal of Endocrinology and Metabolism
