摘要
目的:通过蛋白质组学技术筛查少精子症患者精浆中特异性生物标志物。方法:用H4和SAX-2蛋白质芯片结合SELD I-TOF-MS对20例少精子症患者及20例生育男性精浆样本进行检测,比较两者间蛋白质图谱表达差异。结果:在H4蛋白质芯片上,少精子症患者精浆与生育男性精浆蛋白质图谱相比,有1处蛋白质峰明显增高,差异有显著性(P<0.05),其相对分子质量(Mr)为11 507.4,有1处蛋白质峰明显降低,差异有显著性(P<0.05),其Mr为11 022.9;在SAX-2蛋白质芯片上,少精子症患者精浆与生育男性精浆蛋白质图谱相比有1处蛋白质峰明显增高,差异有显著性(P<0.05),其Mr为16 751.6。结论:少精子症患者精浆中特异性蛋白质的发现,对探究由少精子症引起的男性不育机制及其临床筛查皆具重要意义。
Objective ： To screening specific biomarkers of oligospermic candidates from seminal plasma by proteomics technology. Methods： The protein spectra of 20 seminal plasma from oligospermic patients and healthy male individuals were analyzed by surfaceenhanced laser desorption/ionization time-of-flight mass spectrometry （SELDI-TOF-MS） to compare different protein expressions. Results ： Compared with normal control group, a specific protein peak of molecular weight 11 022.9 in the protein spectra of oligospermic patients＇semen on H4 protein-chip reduced significantly, but the protein peak of molecular weight 11 507.4 elevated apparently. For SAX-2 protein-chip, a specific protein peak of molecular weight 16 751.6 in the protein spectra of oligospermic patients＇semen elevated significantly. Conclusion ： Screening specific proteins in seminal plasma of oligospermic patients is important for understanding the mechanism of oligospermia and screening suspected oligospermic individuals.
出处
《中华男科学杂志》
CAS
CSCD
2006年第1期39-42,共4页
National Journal of Andrology
