摘要
目的:研究中国北方汉族寻常型银屑病(PsV)与HLA等位基因的关联性。方法:采用序列特异性引物-聚合酶链反应(PCR-SSP)方法检测91例PsV患者和102例健康人HLA-A、B、Cw、DRB1及DQB1等位基因。结果:(1)PsV患者HLA-A*0101-03、A*3001-04、B*5701、Cw*0602、Cw*0603/04/05、DRB1*0701/02及DQB1*0201基因频率较正常对照显著增高;Cw*0401基因频率显著下降(Pc<0.05)。(2)I型PsV患者HLA-A*0101-03、A*3001-04、B*5701、Cw*0602、DRB1*0701/02及DQB1*0201基因频率显著增高,而B*51、Cw*0401、DQB1*0301基因频率显著下降;Cw*0603/04/05基因频率在I型及II型PsV患者均显著增高(Pc<0.05)。有家族史PsV患者HLA-A*3001-04、DRB1*0701/02及DQB1*0201基因频率显著增高;无家族史患者Cw*0602基因频率显著增高,而DQB1*0501-04基因频率显著下降(Pc<0.05)。(3)HLA-A*3001-04、DRB1*0701/02及DQB1*0201基因频率仅在男性PsV患者显著增高;B*5701、Cw*0602基因频率仅在女性患者显著增高(Pc<0.05)。结论:(1)HLA-A*0101-03、A*3001-04、B*5701、Cw*0602、Cw*0603/04/05、DRB1*0701/02及DQB1*0201基因可能是北方汉族PsV的易感基因或与易感基因相连锁。(2)HLA-Cw*0401基因可能是阻止北方汉族PsV发病的“保护因子”。(3)I型或II型、有或无家族史PsV在遗传背景上存在差异。
Objective： To investigate the correlation of HLA class Ⅰ and Ⅱ alleles in Northern Chinese Han patients with psoriasis vulgaris. Methods： The polymerase chain reaction amplification with sequence - specific primer （PCR - SSP） method was used to analyse the frequencies of HLA - A, B, Cw, DRB1 and DQB1 alleles in 91 patients with psoriasis vulgaris and 102 healthy controls. Results： （1） The gene frequencies of HLA - A＊ 0101 - 03, A＊ 3001 - 04, B ＊ 5701, Cw＊ 13602, Cw＊ 0603/04/05, DRB1＊ 0701/02 and DQB1＊ 0201 were significantly increased in patients with psoriasis vulgaris than those in normal controls. At the same time, the gene frequency of Cw＊ 0401 was decreased in patients with psoriasis vulgaris, the differentia was significant （ Pc 〈 0.05）. （2） The gene frequencies of HLA - A ＊ 0101 - 03, A＊ 3001 - 04, B＊ 5701, Cw＊ 13602, DRB1＊ 0701/0702 and DQB1＊ 0201 were significantly increased, while the gene frequencies of HLA - B＊ 51, Cw＊ 0401 and DQB1＊ 0301 were decreased in type Ⅰ patients. The gene frequency of HLA - Cw＊ 0603/04/05 was increased in the patients with type Ⅰ and Ⅱ psoriasis vulgaris. The gene frequencies of HLA - A＊ 3001 - 04, DRB1＊ 0701/0702 and DQB1＊ 0201 were more prevalent in patients with family history （Pc 〈 0.05）. The gene frequency of Cw＊ 13602 was increased, but the gene frequency of DQB1 ＊ 0501 - 04 was decreased in patients without family history （ Pc 〈 0.05）. （3） The gene frequencies of HLA - A ＊ 3001 - 04, DRB1＊ 0701/02 及 DQB1＊ 0201 were higher only in male patients with psoriasis vulgaris. In female patients, the gene frequencies of B＊ 5701, Cw＊ 13602 were higher, the differentia was significantly （ Pc 〈 0. 05）. Conclusion： （1）HLA-A＊101-03, A＊001-04, B＊5701, Cw＊0602, Cw＊ 0603/04/05, DRB1＊ 0701/ 0702 and DQB1 ＊ 0201 alleles may be suseeptibihty genes, or in close Iinkage with the susceptibility genes of psoriasis vulgaris in Northern Chinese Hart. （2） HLA - Cw＊ 0401 allele seems to be a protective factor against the development of psoriasis vulgaris. （3） There may be a difference in genetic background between patients with type Ⅰ and type Ⅱ, with and without family history of psoriasis vulgaris.
出处
《中国麻风皮肤病杂志》
2006年第1期1-4,共4页
China Journal of Leprosy and Skin Diseases
基金
美国China Medical Board基金(Grant#00-734)
