摘要
采用多元酸酐法活化盐酸莱克多巴胺,用混合酸酐法将活化的莱克多巴胺与载体蛋白(KLH,BSA)偶联,制备了莱克多巴胺免疫原KLH-Rac和包被抗原BSA-Rac。以KLH-Rac免疫新西兰白兔获得了莱克多巴胺多克隆抗体,以BSA-Rac为包被抗原,采用间接ELISA检测抗血清,其效价达到1∶6 000,间接竞争ELISA测得抗血清的IC50值约为5 ng/mL,其与克伦特罗、沙丁胺醇、特布它林的交叉反应性小于0.01%。
Ractopamine HC1 was activated by the multi-anhydride method and the activated ractopamine was conjugated to a carrier protein by the mixed-anhydride method. The carrier protein was keyhole limpet hemocyanin（KLH） and bovine serum albumin（BSA）. The KLH conjugate（KLH-Rac） was used as immunogen and the BSA conjugate（BSA-Rac） was used as the coating antigen for the development of an ELISA. New Zealand rabbits were immunized with KLH-Rac. Titer of the polyclonal antiserum detected by the developed indirect ELISA method was more than 6,000. The antiserum IC50 detected by an indirect competitive ELISA was approximately 5 ng/mL. The cross reactivity with clenbuterol,salbutamol and terbutaline was less then 0.01%.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2006年第1期62-65,共4页
Chinese Veterinary Science
基金
农业部"948"项目(2003-Q08)
