摘要
目的探讨内皮素(ET)在梗阻性黄疸肝、肾、心组织损伤中的作用机理及血塞通注射液的保护作用。方法健康日本大耳白兔72只,雌雄各半,采用完全随机分组方法随机均分为3组:胆总管结扎(BDL)组、BDL+血塞通注射液组及假手术组。建立实验动物模型,分别于术后3、6、9及21d取材。用全自动生化分析仪测定各组动物血清总胆红素、谷丙转氨酶、尿素氮及肌酐;用放免法测定各组动物肝、肾、心组织及血浆中的ET水平。结果BDL组和BDL+血塞通注射液组与假手术组比较,动物血浆及肝、肾、心组织中的ET含量明显升高(P<0.01),肝、肾功能损害更严重(P<0.01);BDL+血塞通注射液组比BDL组血浆及组织中ET含量明显降低(P<0.05),肝、肾功能损害亦减轻(P<0.05)。结论梗阻性黄疸可导致血浆及肝、肾、心组织ET含量升高,且随梗阻时间延长升高愈明显;血塞通注射液通过降低血浆及组织的ET水平,发挥对梗阻性黄疸肝、肾、心损害的保护作用。
Objective To study the effects of endothelin （ET） and Xuesaitong injection on hepatic, renal and myocardial tissues after bile duct ligation （BDL） in rabbits. Methods Seventy-two rabbits were randomly divided into three groups; BDL group （24 rabbits）, BDL＋Xuesaitong injection group （24 rabbits）, and sham operation group （24 rabbits）. Each group was subdivided into four subgroups of postoperative 3, 6, 9 and 12 d （6 rabbits in each subgroup）. Automatic biochemical analysis equipment was used to detect the levels of serum TBIL, ALT, BUN and Crea. The levels of ET in plasma, hepatic, renal and myocardial tissues were measured with radioimmu nological method. Results The levels of ET in plasma, hepatic, renal and myocardial tissues in both BDL group and BDL＋Xuesaitong injection group were higher than those of sham operation group （P〈0.01）. The levels of ET in plasma and tissues of BDL＋Xuesaitong group were lower than those in BDL group （P〈0.05）. Conclusion Obstructive jaundice can lead to an increase of ET in plasma, hepatic, renal and myocardial tissues, the level of ET in creases with the time of obstruction. Xuesaitong injection may play a protective role in the iniury of hepatic, renal and myocardial tissues after obstruction by decreasing the level of ET in plasma and tissues.
出处
《中国普外基础与临床杂志》
CAS
2006年第1期77-81,共5页
Chinese Journal of Bases and Clinics In General Surgery
关键词
梗阻性黄疽
内皮素
血塞通注射液
兔
Obstructive jaundice Endothelin Xuesaitong injection Rabbit
