摘要
为了阐明脾虚证模型大鼠血浆中β-endorphin,β-EP)、降钙素基因相关肽(calctonin gene related peptide,CGRP)和神经肽Y(neuropeptide Y,NPY)变化规律和健脾益气方剂四君子汤对其的调节作用以及对脾虚证病理过程的保护机制,应用利血平复制出脾虚证大鼠模型,以特异性放射免疫分析法检测了血浆β-EP、CGRP和NPY的含量变化。结果显示,脾虚证模型大鼠血浆β-EP、CGRP比对照组显著升高(P<0.01),NPY比对照组显著降低(P<0.01),接近对照组水平。提示脾虚证的病理变化过程与β-EP、CGRP、NPY等神经肽类的失衡有一定的相关性,健脾益气方剂四君子汤对脾虚证模型大鼠血浆内β-EP、CGRP和NPY含量变化有较好的调节作用,推测这也许是四君子汤健脾益气能够治疗脾虚证的作用机制之一。
The rat spleen dificiency syndrome models was cleveloped by administration of reserpine 1 mg/（kg·d） for seven days to explore the relationship among β-endorphin,calcitonin gene related peptide ,neuropeptide Y and pathogenesis of Spleen deficiency syndrome in traditional Chinese medicine. The β-EP,CGRP and NPY levels in plasma of rat models were measured by radioimmunoassay（RIA） ,and compared with the normal group and the group treated with Sijunzi decoction. The results indicated that in rat spleen dificiency model both the contents of β-EP and CGRP in plasma increased（P〈0. 01 ）,while the level of NPY in plasma decreased （P〈0.01）. Meanwhile after Sijunzi decoction treatment,the contents of β-EP,CGRP and NPY were all recovered. The results showed that there were obvious imbalance of β-EP,CGRP and NPY in rat model of spleen deficiency syndrome and the Sijunzi decoction could correct it ,which may be one of the mechanism for treatiment of the spleen dificiency syndrome.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2006年第1期85-87,共3页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(39770558)
