摘要
目的探讨非酒精性脂肪性肝炎(nonalcoholsteatohepatitisNASH)大鼠血管内皮细胞分泌功能的变化在其发病机制中的意义。方法健康雄性Wistar大鼠16只,随机分为正常组和模型组,分别以标准大鼠饲料和高脂饲料喂养,连续12周后处死大鼠,HE染色观察大鼠肝脏组织病理学变化,脂酶法检测肝组织匀浆TG、CHOL含量,黄嘌呤氧化酶法检测血及肝组织匀浆中SOD含量,TBA法检测血及肝组织匀浆中MDA含量,放免法测定血浆ET、TXB2、6-Keto-PGF1α含量,发色底物法测定血浆t-PA、PAI含量,硝酸还原酶法检测血清NO含量。结果模型组大鼠肝组织均有重度脂肪变,并伴有肝细胞气球样变,肝细胞碎屑样坏死、炎症细胞浸润和汇管区渗出,未见肝纤维化和肝硬化形成。模型组大鼠肝组织匀浆TG、CHOL含量较正常组明显升高(P<0.01);血清及肝组织匀浆中MDA含量较正常组明显增高(P<0.05),SOD活力均较正常组明显下降(P<0.05);血ET、NO、TXB2、6-Keto-PGF1浕、PAI含量以及ET/NO、TXB2/6-Keto-PGF1α、PAI/t-PA值均较正常组明显升高(P<0.01,P<0.05);t-PA水平与正常组相比差异无显著性(P>0.05)。结论高脂饲料可成功诱导大鼠非酒精性脂肪性肝炎;大鼠在非酒精性脂肪性肝炎时出现了血管活性因子分泌的紊乱,表明非酒精性脂肪性肝炎存在着血管内皮的损伤。
Objective To study the significance of vascular Endothelial cell＇ s secretion function in the pathogenesis of NASH rats, Method 16 healthy male Wistar rats were randomly divided into normal group and modle group and fed with standard and high - fat diets, then were put to death after 12 weeks.Then the alteration of hemorheology and the contents of liver TG, CHOL, serum ALT, AST were detected.The pathological change of liver tissues were observed by HE staining, the SOD activity of liver tissues were detected with xanthione oxidase assay. The contents of liver MDA were detected with TBA assay, the contents of ET, TXB2, 6 - Keto - PGF1α were detected with radioimmunoassay.The contents of serum NO were detected with nitre reductase, and the contents of t- PA, PAl were detected too.Results The rat liver tissues in the motile group all showed severe fatty change, inflammatory cells infiltration, hepatcyte necrosis, portal area exudation.The contents of liver TG, CHOL in modle group all increased significantly compared with normal group（ P 〈 0.01）. Liver and senun MDA all increased significantly compared with normal group （ P 〈 0.05）. The SOD activity were lower（ P 〈 0.05）. The contents of ET, NO, TXB2, 6 - Keto - PGF1α, PAI and the ratio of ET/NO, TXB2/6 - Keto - PGF1α, PAL/t - PA in modle group all increased significantly compared with the normal group （ P 〈 0.01, P 〈0.05）, the level oft - PA changed insignificantly compared with normal group （P 〉 0.05） .Conclusion High- fat diet may result in nonalcohol steatohepatitis successfully; the inodle rats of nonalcohol steatohepatitis show secreting disorder of vascular active factors, which indicate NASH rats have the injury of vascular endothelial cell.
出处
《医学研究杂志》
2006年第2期12-15,共4页
Journal of Medical Research
关键词
非酒精性脂肪性肝炎
血管活性因子
内皮细胞
Nonalcohol steatohepatitis
Vascular active factors
Vascular endothelial cell
