摘要
槲皮素对培养内皮细胞产生和释放内皮素和环磷酸鸟苷的影响赵雪英，顾振纶（苏州医学院药理学教研室，苏州２１５００７，中国）关键词槲皮素；脐静脉；血管内皮；培养的细胞；凝血酶；血小板；内皮素；环鸟苷一磷酸目的：研究槲皮素（Ｑｕｅ）对不同状态下的内皮细胞形成...
IM: To evaluate the effects of quercetin (Que)
on the formation and release of endothelin (ET) and cyclic guanosine
monophosphate (cGMP) by endothelial cells. METHODS: Radioimmuno￣
assay (RIA) was used to assess the amounts of ET and cGMP produced
by unstimulated human umbilical vein cells (HUVEC) or HUVEC
stimulated with activated platelets and thrombin. RESULTS: Following
the treatment of the cultured HUVEC with activated platelets, the
amount of ET in the medium was increased, but no significant effect
on cGMP within the cells was seen. Thrombin not only increased the
amount of ET in the medium, but also raised the level of cGMP within
the cells. Pretreatment of HUVEC with Que 0 5, 5, and 50 μmol·L
-1 , the release of ET by HUVEC stimulated with thrombin and
thrombin activated platelets was much inhibited. Que 0 5-50 μ
mol·L -1 markedly increased the amount of cGMP in HUVEC
stimulated with thrombin and activated platelets. Que also inhibited
the release of ET from unstimulated HUVEC, but increased the
production of cGMP from unstimulated EC. CONCLUSION: Thrombin and
activated platelets stimulated the release of ET from HUVEC and Que
inhibited the release of ET and promoted the formation of cGMP from
HUVEC.
出处
《中国药理学报》
CSCD
1996年第5期442-444,共3页
Acta Pharmacologica Sinica
关键词
槲皮素
内皮细胞
凝血酶
内皮素
环鸟苷
磷酸
quercetin
umbilical veins
vascular endothelium
cultured
cells
thrombin
platelets
endothelins
cyclic GMP
