摘要
目的探讨慢性阻塞性肺疾病(COPD)炎症发生的分子生物学机制。方法对12例COPD患者(COPD组)、10例支气管哮喘患者(哮喘组)、10例健康成年人(对照组)采用免疫细胞化学和逆转录-聚合酶链反应技术,检测其外周血单个核细胞(PBM C)中转录因子激活蛋白AP-1(c-fos和c-jun)及白细胞介素8(IL-8)的表达水平。结果c-fos表达在COPD组和哮喘组之间无显著性差异,但均高于对照组(P<0.05)。c-jun、IL-8的表达在COPD组明显高于其他两组(P<0.05),且c-jun与IL-8的表达呈明显正相关。结论c-jun的高表达在COPD的慢性炎症过程中具有重要作用,高表达的c-jun可能使AP-1活性增强,促进IL-8表达升高。
Objective： To investigate the possibly molecular mechanism of inflammation in chronic obstructive pulmonary disease （COPD）. Methods： Peripheral venous blood were collected from 12 patients with COPD, 10 patients with asthma, 10 healthy cases, and peripheral blood monoenuclear cells （PBMC） were extracted with ficioll-hypague. The mRNA levels of c-los, c-jun, IL-8 were evaluated by reverse transeription-polymerase chain reaction （RT-PCR）. The protein levels of c-fos, c-jun were determined by immunoeytoehemistry （ICC）- Results ： The c-fos level in COPD group and the asthma group had no differenee, but were both higher than that in the control group. The levels of c-jun and IL-8 in COPD group were significantly higher than that in the asthma group and the control group （P〈0. 05）. The expression of c-jun had a positive correlation with IL-8 （P d0. 05）. Conclusions： The increased expression of c-jun playes an important role in COPD, it may enhance the activity of AP-1 and increase the expression of IL-8.
出处
《山东医药》
CAS
北大核心
2006年第4期3-5,共3页
Shandong Medical Journal
基金
安徽省自然科学基金资助项目(03043706)
