摘要
为探讨P物质(SP)和Fos蛋白与哮喘发病的关系,本实验采用免疫组织化学方法及计算机图像分析技术对正常对照大鼠和哮喘大鼠肺内SP免疫阳性纤维的分布变化及Fos蛋白的表达情况进行了研究。结果显示:(1)正常对照组大鼠肺组织内可观察到SP阳性纤维分布于支气管至终末细支气管和肺血管壁内,但呼吸性细支气管和肺泡壁内少见SP阳性纤维。哮喘组大鼠肺内SP阳性纤维的数量及分布均发生了明显变化,从肺内支气管到终末细支气管均有较密集的SP阳性纤维呈束走行;且呼吸性细支气管和肺泡壁内也出现了SP阳性纤维。哮喘组大鼠肺内SP阳性纤维的数量明显高于正常对照组大鼠(P<0.05)。(2)正常对照组大鼠的肺内几乎不表达c-fos基因,但在哮喘组大鼠肺内可观察到许多Fos阳性细胞,这些细胞主要为炎性细胞和平滑肌细胞。以上结果表明,肺内SP阳性纤维增生及c-fos基因表达与哮喘发病密切相关。
To investigate the relationship of substance P （SP） and Fos-protein with asthmatic disease, the changes of SP-immunoreactive （SP-IR） fibers and expression of Fos-positive neurons were compared between normal control rats and asthma rats by means of immunohistochemical method combined the computer image analysis. The results were as following： （ 1 ） SP-IR fibers were observed on the walls from the bronchi to terminal bronchioles, and the walls of blood Vessels of lungs of normal control rats. But SP-IR fibers on the bronchioles and pulmonary alveoli were seldomly. However, it was detected that obvious changes in the distribution and numbers of SP-IR fibers in airways of asthma group. These SP-IR fibers were observed on the walls of brouehi and terminal bronchioles, emerging as large fiber bundles, and on respiratory bronehioles and pulmonary alveoli. The numbers of SP-IR fibers in asthma group were significantly greater than normal control group（ P 〈0.05 ） ; （2） The Fos-positive cells were not observed in the lungs of normal control rats, while expression of Fos-protein were found in the inflammatory cells and smooth muscle cells of asthmatic lungs. The present results suggest that the hyperplasia of SP-IR fibers and expression of Fos-protein in the lung are closely related to the development of asthma.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2005年第5期526-530,共5页
Chinese Journal of Neuroanatomy
