摘要
通过对不同化工厂废水处理池的活性污泥进行富集、不同浓度银离子驯化后,分离得到30株银离子抗性细菌,最大硝酸银耐受量可达80mg.ml-1.对这些细菌进行质粒抽提与鉴定,质粒检出率为76.67%.40mmol.L-1的苯甲酸钠对HAg4细菌质粒的消除率可达98.75%;而350μg.ml-1的吖啶橙对HAg4细菌质粒的消除率只有77.78%.细菌质粒与银离子抗性密切相关.
Through the enrichment of the active mud obtained from three chemical plants and the domestication with different concentration Ag^＋ solution, thirty bacteria strains with silver （Ag^＋）-resistance were isolated, among which, the highest Ag^＋ -resistant concentration was 80 mg·ml^-1, The plasmids in these bacteria were extracted, with the detection rate of 76.67 %. The elimination rate of the plasmid in HAg4 bacteria was 98.75 % by 40 mg·ml^-1 sodium benzoate, and 77.78 % by 350 μg·ml^-1 acridine orange. It was suggested that the Ag^＋ -resistance of bacteria was highly correlated with their plasmids.
出处
《应用生态学报》
CAS
CSCD
北大核心
2006年第2期305-308,共4页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(30471378)
浙江省自然科学基金项目(Y504256)
浙江省教育厅高校科研计划资助项目(20050058).
关键词
细菌
质粒
银离子
抗性
Bacteria, Plasmid, Silver, Resistance.
