摘要
应用离心淘洗法同步化细胞研究3种抗癌药在体外培养细胞中细胞毒作用的时相特异性。结果表明,在同步化的V79细胞中G_2M细胞对安吖啶最敏感,S细胞居中,G_1细胞相对敏感性较低。同步化的P388细胞对小红参醌的敏感性顺序和安吖啶相类似。同步化的L1210细胞对平阳霉素的敏感性顺序为G_1细胞最敏感,G_2M细胞居中,S细胞敏感性最低。此外,还发现戊脉安对平阳霉素及安吖啶的细胞毒性均有明显增效作用,且这种增效作用也具有明显时相特异性。应用流式细胞光度术研究了上述药物对细胞周期进程的影响,结果证明3种抗癌药均可使细胞周期阻断于G_2M时相。戊脉安可明显增强平阳霉素的G_2M阻断作用。
V79 ,L1210 and P388 cells were synchrnonized by centrifugal elutriat-ion.The study of amsacrine(AMSA)cytotoxicity in synchronized V79 cells showed that G2M cells were most sensitive to AMSA, with S cells intermediate and G1 cells resistant. In synchronized L1210 cells the Gl cells were most sensitive to the cytotoxicity of Pingyang-mycin, with G2M cells intermediate and S cells resisitant.The study of Rubiquinone cytotoxicity in synchronized P388 cells showed that the order of drug sensitivity was similar to that of AMSA. The phase-dependent cytotoxicity of AMSA and pingyangmycin could be enhanced by verapamil and the enhancement ratio was also phase-dependent. DNA distribution patterns measured by flow cytometry showed that a significant G2M block could be obtained in V79, L1210 and P388 cells exposed to AMSA, pingyangmycin or Rubiquinone.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1990年第3期200-204,共5页
Chinese Journal of Cancer
基金
国家自然科学基金
