摘要
A new method has been developed for separation and determination of zinc,copper,cobalt and nickel based on their colour chelates with 6,6″-dimethyl-4′-phenyl-2,2′:6′,2″-terpyridine（TPY） by ion pair high performance liquid chromatography in a reversed phase mode.The suitable conditions are the methanol-water（80:20,V/V） containing 10mmol/L NaH2PO4-H3PO4 buffer solution（pH=5.0） and 3.0mmol/L sodium dodecyl sulfonate as mobile phase,on a Symmetry C18 column（3.9mmID×150mm）,the flow rate of the mobile phase is 1.0mL/min.Spectrophotometric detection was made at 340nm.The detective limits are 0.05,0.10,2.00 and 0.50ng/mL respectively.The method has good selectivity and sensitivity.It has been applied to the separation and determination of zinc,copper,cobalt and nickel in human hair and tobacco and the result is satisfied.
A new method has been developed for separation and determination of zine,copper,eobalt and nickel based on their colour chelates with 6,6＂ - dimethyl - 4＇ - phenyl - 2,2＇ ：6＇ ,2＂ - terpyridine （TPY） by ion pair high performance liquid chromatography in a reversed phase mode. The suitable conditions are the methanol- water （80：20, V/V） containing 10mmol/L NaH2 PO4 - H3PO4buffer solution （pH = 5.0） and 3.0mmol/L sodium dodecyl sulfonate as mobile phase, on a Symmetry C18 column （3. 9tumid × 150mm） ,the flow rate of the mobile phase is 1.0mL/min. Spectrophotometric detection was made at 340nm. The detective limits are 0. 05,0. 10,2. 00 and 0. 50ng/mL respectively. The method has good selectivity and sensitivity; It has been applied to the separation and determination of zinc,copper,cobah and nickel in human hair and tobacco and the result is satisfied.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2006年第2期189-191,共3页
Chemical Research and Application
