摘要
目的:检测人胃癌及相应癌旁组织中法尼基转移酶(FTase)β-亚单位mRNA表达和H-ras基因第12密码子点突变探讨FTaseβ-亚单位基因在胃癌中的表达与临床病理特点、H-ras基因突变的关系.方法:收集胃癌及相应癌旁正常组织标本43例.应用半定量逆转录酶-聚合酶链反应(RT-PCR)测定FTaseβ-亚单位mRNA基因表达水平,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析H-ras基因第12密码子点突变情况.采用秩和检验分析胃癌和癌旁组织的FTaseβ亚单位mRNA表达的配对数据的差异;采用多元逐步回归分析胃癌组织中FTaseβ亚单位mRNA表达活性与临床病理特点及H-ras基因突变间的关系.结果:胃癌组织中FTaseβ亚单位mRNA的平均值明显高于癌旁组织(0.89±0.48vs0.69±0.40),两者相比有显著性差异(Z=2.469,P=0.014).在43例胃癌组织中有1例发现突变(1/43,2.3%),癌旁组织中未发现突变.胃癌组织的FTaseβ亚单位mRNA表达活性与年龄、肿瘤部位、组织学分级、有无淋巴结转移及有无H-ras基因突变无关,在女性,病理分型为印戒细胞癌的胃癌组织中有较高的FTaseβ亚单位mRNA表达活性.结论:FTaseβ亚单位mRNA在胃癌组织中表达升高.胃癌组织中H-ras基因第12密码子点突变率低,在胃癌的发生中不起主要作用,并且与FTaseβ亚单位mRNA的表达无相关.
AIM： To investigate the expression of farnesyltransferase（FTase） beta-subunit mRNA in gastric cancer tissues and its relations with the clinicopathological features and point mutation in codon 12 of H-ras gene.
METHODS： Specimens were collected from 43 cases of gastric cancer and their corresponding normal tissues. The expression of FTase beta-subunit mRNA was investigated by semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR）. The point mutation in codon 12 of H-ras gene was detected by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Wilcoxon Signed-Rank Test was used to analyze the difference of the matching data of FTase beta-subunit mRNA expres- sion between gastric cancer and normal tissues. Multiple linear regression analysis was used to explore the relations of FTase activity with the clinicopathological features and H-ras mutation in gastric cancer
RESULTS： The mean level of FTase beta-subunit mRNA expression were significantly higher in gastric cancer tissues than that in the corresponding normal ones（0.89 ＋ 0.48 vs 0.69 ＋ 0.40, Z= 2.469, P = 0.014）. Of the 43 cases, the mutation of H-ras 12 codon was found in 1 case of cancer tissue（1/43, 2.3%）, and no mutation appeared in the all the normal tissues. The expression of FTase beta-subunit mRNA was not related to the age, tumor location, histological differentiation, lymph node metastasis and H-ras 12 codon mutation. But the female patients with signetring cell carcinoma had higher expression of FTase beta-subunit mRNA. CONCLUSION： The expression of FTase beta-subunit mRNA is up-regulated in gastric cancer. The point mutation in codon 12 of H-ras gene is not related to the expression of FTase beta-subunit mRNA.
出处
《世界华人消化杂志》
CAS
北大核心
2005年第21期2565-2569,共5页
World Chinese Journal of Digestology
