摘要
采用ＰＣＲ、ＰＣＲ－ＲＦＬＰ等方法检测５１例宫颈组织（其中宫颈癌标本３８例，正常宫颈组织标本１３例）中高危型人乳头瘤病毒（ＨＰＶ）－１６，１８感染情况及Ｈ－ｒａｓ基因突变情况。研究结果显示，宫颈组织中ＨＰＶ－１６，１８感染率为７４％（２８／３８），表明高危型ＨＰＶ－１６，１８是宫颈癌发生的重要因素。Ｈ－ｒａｓ基因突变率为２９％（１１／３８）。正常宫颈组织中上述检测结果均为阴性。Ｈ－ｒａｓ基因突变与多种肿瘤发生有关；显示该基因突变与宫颈癌发生有关，但不是早期事件，也不能作为临床恶变与预后判断的指标；Ｈ－ｒａｓ基因突变与ＨＰＶ－１６。
Mutation of certain oncogene, including C-myc H-ras CerbB-2, have been associated with many kinds of tumor and have been implicated in their natural history. But the relationship of these gene alteration to cervical cancer with or without HPV16, 18 infection has not studied clearly. Cervical tissue DNA from 13 normal controls and 38 patients with biopsyproven cervical cancer were analyzed for the presence of human papillomavirus (HPV) type 16, 18 and H-ras mutation. HPV types 16, 18 were detected in 28 of 38 patients, the positive proportion is 74%. However only 1 mutation of H-ras in normal controls was found. It suggests that HPV16, 18 infection is a high-risk factor in cervical cancer. However occurrence mutation of H-ras oncogene of in cervix bears no clinical significance.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
1998年第4期289-292,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省教委科研基金
