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氯化镉对人肝癌细胞SMMC-7721抑制作用 被引量：8

Inhibition effect of cadmium chloride on human hepatocellular carcinoma cells SMMC-7721
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摘要 目的研究氯化镉(CdCl2)对人肝癌细胞(SMMC-7721)的抑制作用并探讨其作用机制。方法应用生长曲线法、四甲基偶氮噻唑蓝法(MTT)、流式细胞术(FCM)检测CdCl2对SMMC-7721的生长抑制及对细胞周期的影响。结果CdCl2对肝癌细胞SMMC-7721有明显的生长抑制作用,且存在剂量-效应关系;影响细胞周期进程并出现G0/G1期阻滞。结论CdCl2能有效抑制肝癌细胞株的生长,其抑制作用可能与影响细胞周期进程有关。 Objective To study the inhibition effect of cadmium chloride（CdCl2 ）on detected by growth curve and methyl thiazolyl tetrazolium（MTT） assay, and cell cycle was determined by flow cytometry（FCM）. Results CdCl2 showed inhibition effect on the proliferation of SMMC-7721 in dose-dependent manner. Cell cycle was arrested in G0/G1 phase. Conclusion CdCl2 possesses growth inhibition effect on hepatocellular carcinoma cells and the mechanism may be related to cell cycle arrested.
作者 杜海英 刘晓梅 刘颖 金明华 黄渭 孙志伟
机构地区 吉林大学公共卫生学院卫生毒理教研室
出处 《中国公共卫生》 CAS CSCD 北大核心 2006年第2期194-195,共2页 Chinese Journal of Public Health
基金 国家教委博士点基金
关键词 氯化镉 人肝癌细胞 细胞周期 SMMC-7721 抑制作用 cadmium chloride hepatocellular carcinoma cell cell cycle
分类号 R735.7 [医药卫生—肿瘤]
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参考文献6

  • 1Micheal PW, Bhalchandar AD, Sabin R, et al. Down - regulation of MT expression in human and murine hepatocellular tumors:associations with the tumor - necrotizing and antineoplastic effects of cadmium in miee[J]. Pharm Exp Thrapeutics, 1996, 277(2) : 1026 -1033.
  • 2卢国栋,金泰廙.镉对细胞凋亡的双重作用[J].环境与职业医学,2001,18(5):299-301. 被引量：8
  • 3Deng DX, Cai L, Chakrabarti S, et al. Increased radiation - induced apoptosis in mouse thymus in the absence of metallothionein [J].Toxicology, 1999, 134(1) :39 - 49.
  • 4Piersma AH, Roelen B, Roest P. Cadmium - induced inhibition of proliferation and differentiation embryonal carcinoma cell and mechartistic aspects of protection by zinc [J]. Teratology, 1993, 48 (4):335-341.
  • 5Holt D, Webb M. Teratogenicity of ionic cadmium in the wistar rat[J]. Arch Toxicol, 1987, 59(6) :443 - 447.
  • 6Johnson DG, Walker CL. Cyclins and cell cycle checkpoints[J]. Annu Rev Pharmacol Toxicol, 1999, 39:295 - 312.

二级参考文献19

  • 1[1]John D.Robertson and Sten Orrenius.Molecular mechanisms of apoptosis induced by cytotoxic chemicals[J].Critical Review in Toxicology.2000,30(5):609-627.
  • 2[3]E1 Azzouri B,Tsangaris GT,Pellegrini O,et al.Cadmium induces apoptosis in a human T cell lines[J].Toxicology,1994,88(1-3):127-389.
  • 3[4]Matsuoka M,Call KM.Cadmium-induced expression of immediate early genes in LLC-DK1 cells[J].Kidney Int,1995,48(2):383-389.
  • 4[5]Hamado T,Tanimoto A,Iwal S,et al.Cytopathological changes induced by cadmium-expossure in canine proxima;tubular cells:a cytochemical and ulttrastructureal study[J].Nephron,1994,68(1):104-111.
  • 5[6]Xu GG,Zhou GO,Jin TY,et al.Apoptosis and P53 gene expression in male reproductive tissues of cadmium exposed rats[J].Biometals,1998,12:131-139.
  • 6[8]Achanzer WE,Achanzar KB,Lewis JG,et al.Cadmium induces c-myc and c-jun expression in Human Prostate Epithelial Cells as a prelude as a prelude to apoptosis[J].Toxicol Appl Pharmacol,2000,164(3):291-300.
  • 7[9]Ding W,Templeton DM.Activation of parallel mitogen-activated protein kinase cascades and induction of c-fos by cadmium[J].Toxicol Appl Pharmacol,2000,162(2):93-99.
  • 8[10]Yuan C,Kadiiska M,Achanzar WE,et al.Possible role of caspase-3 inhibition in cadmium-induced blockage of apoptosis[J].Toxicol Appl Pharmacol,2000,164(3):321-329.
  • 9[11]Chen G,Brandon PE,Shore GC.Induction of P-53 independent apoptosis by hygromycin b:Suppresion by Bcl-2 and adenovivus E1B 19-kDa protein[J].Exp Cell Res,221(1):55-59.
  • 10[12]Kasai T,Ohguchi K,Nakashima S,et al.Increased activity of oleate-dependent type phospolopase during actinomycin D-induced apoptosis in Jurkat T cells[J].J Immunol,161(12):6469-6474.

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中国公共卫生
2006年 第2期

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