摘要
嗜硫色谱在高盐环境下对抗体及其他某些蛋白质产生特异性吸附,再在低盐条件下洗脱,可获得高纯度及高回收率的蛋白质产品,是一种新型蛋白质纯化技术。该文对嗜硫色谱及其在抗体纯化中的应用做了综述。
Thiophilic adsorption chromatography binds antibodies and other proteins in the presence of high concentration of salt. When eluted by decreasing the salt concentration, the high purity and recovery of proteins can be achieved. It is a new chromatographic technology for the purification of proteins. The review introduces the method for the purification of antibodies and covers the development and application of the original technology.
出处
《色谱》
CAS
CSCD
北大核心
2006年第1期88-92,共5页
Chinese Journal of Chromatography
基金
陕西省教育厅科研计划项目(项目编号:02JCK114)
关键词
嗜硫吸附
色谱
抗体纯化
thiophilic adsorption
chromatography
antibody purification
