摘要
采用电泳技术分析了三峡地区１０份玉米地方品种和４个外来种质在１６个同工酶标记位点上的遗传变异。结果表明，在所有材料中存在广泛的同工酶变异。１０个地方品种中含有外来种质所没有的Ｇｏｔ２－６基因，但却没有外来种质中所含有的Ｅｓｔ８－４．５、Ｍｄｈ２－５．５和Ｓｏｄ１－Ｂ三个等位基因。地方品种与外来种质之间基于１６个位点所估算的ＭＲＤ值在０．１４８—０．３９１之间，地方品种间ＭＲＤ值在０．１１０－０．４４４０之间。每一标记位点的ＭＲＤ值与Ｆ１代数量性状表现的相关分析表明，１Ｌ的Ａｃｐ４、Ａｄｈ１附近的染色体区段，可能直接或间接地影响产量，但是基于１６个位点估算的地方品种与外来种质的ＭＲＤ值与ＳＣＡ、ＳＧＤ与ＳＣＡ、ＧＧＤ与ＧＣＡ相关不显著，说明应用少数同工酶的遗传多样性对杂种优势预测的可靠性是有限的。
Ten local varieties collected from thethree gorges area and four exotic populations of maize (Zea mags L.) were assayed for allozyme variation at 16 enzyme marker locus. Extensive allozyme variability was observed in all populations studied, 1.69 and 1.75 alleles per locus werepresented within per local variety and exotic population respectively. Got2-6 was presented within ten tocal varieties hut was not discovered in exotic populations Est8-4.5、Mdh2-5.5 and Sod1-B were observed in four exdtic population but were not observed in local varieties. MRD (Modified Rogers Distance) based on 16 locus had revealed the genetic difference among local varieties (0.148￣0.391) and between local varieties and exotic populations (0.110￣ 0.444). The correlation coefficient between MRD based on per se isozyme loci and hybrid performence of quantitative traits showed that chromosome fragment near Acp4 and Adh1 on the 1L associated with yield per plant directly or indirectly. But there were no relationship between MRD based on 16 allozyme locus and SCA (Speircific Combining Ability) and between SGD (Sperific Genetic Distance) and SCA of yield and other quantitative traits. The results indicated allozyme marker loci were of limited usefulness in predicting heterosis in maize.
出处
《湖北农学院学报》
1996年第1期1-8,共8页
Journal of Hubei Agricultural College
基金
国际科学资金
国家自然科学基金
关键词
玉米
地方品种
同工酶
遗传多样性
数量性状
Maize (Zea Mays L.)
Local Variety
Allozyme
Genetic Diversity
Quantitative trait
