摘要
为了了解禽流感病毒在黑龙江大庆地区禽类中的流行情况,我们在大庆地区五区四县采集了1180份样品,采用常规鸡胚尿囊腔接种方法分离病毒,利用微量血凝试验(HA)及RT-PCR鉴定。结果在大庆地区所采集的样品,未检测出有血凝活性;而在绥化地区采集样品中检测出3株禽流感病毒。虽然大庆地区未检测出禽流感病毒,但在周边的绥化地区检测出禽流感病毒,大庆市有被禽流感传染的威胁,提示应加强对禽流感的预防。
1180 samples were collected from poultry farms in Daqing area in order to exam the distribution of avian influenza. Virus isolation was performed using the embryonated eggs with routine method. Avian influenza virus was detected with micro-hemagglutination （HA） test and RT-PCR. The Results showed that no hemagglutination activity was detected in the samples, but three strains of avian influenza virus were detected by RT-PCR from Suihua area. It indicated that Daqing had been threatened by avian influenza, although there was no avian influenza in Daqing area, and the prevention of avian influenza should be focused and intensified.
出处
《黑龙江八一农垦大学学报》
2005年第6期42-44,共3页
journal of heilongjiang bayi agricultural university
基金
大庆市科技局资助。
