摘要
采用聚合酶链式反应克隆暗腹雪鸡（Tetraogallus himalayensis）Cytb基因，首次报道该基因的全长序列（1143bp AY678108），并与雉科中其他19个属的Cytb基因进行同源性比较，分析了碱基组成和变异情况．用邻接法、最大简约法和最大拟然法构建了系统进化树，得到了基本相同的拓扑结构．结果表明雪鸡属与鹌鹑属、石鸡属的亲缘关系最近，形成单系群．雪鸡属与鹌鹑属的分歧时间为1．5～2．0百万年，与石鸡属分歧时间为1.7～2．4百万年．
Up to now, there were few references about Tetraogallue, especially in its phylogenetic relationships with other genera in Phaslanidae. The complete mitochondrial cytochrome b gene of Tetraogallus himalayensis was amplified by PCR. DNA fragment of 1143bp（AY678108） was reported for the first time. By comparing with the sequence of other nineteen genera in Phasianidae, the base compositions and divergences among these sequences were analyzed by MEGA software, the phylogenetic trees constructed by multiple methods（NJ ,MP,ML） suggested the same topology. The molecular results revealed that Tetraogallue has the most closed relationship with Coturnix and Alectoris. These three genera are monophyletic. The approximate divergence time estimated by base change velocity between Tetraogallue and Coturnix was 1.5 - 2.0 million years. The divergence time between Tetraogallue and Alectoris was 1.7-2.4 million years.
出处
《浙江大学学报（理学版）》
CAS
CSCD
北大核心
2006年第1期89-94,共6页
Journal of Zhejiang University（Science Edition）
