摘要
采用聚合酶链式反应,从丹顶鹤(G.joponensis♂)、白枕鹤(G.vipio♀)、灰鹤(G.grus)及丹顶鹤♂和白枕鹤♀的4个杂交子代中分别扩增出线粒体DNA细胞色素b基因,并测定出细胞色素b碱基序列,它们之间的序列差异在0.1%～7.2%之间.序列分析表明几种鹤类细胞色素b基因序列的碱基变化主要是转换和颠换,转换的比率大于颠换;绘制几种鹤类细胞色素b之间的亲缘关系进化树表明丹顶鹤和灰鹤的进化距离更近,4个杂交子代与母本白枕鹤的亲缘关系更近.
Using PCR techniques the fragments of the Cytochrome-b were amplified and sequenced from G.joponensis (♂)、G.vipio(♀)、G.grus and four interspecific F_1 hybrids between G.joponensis(♂) and G.vipio (♀).The sequence analysing of Cytochrome-b from several cranes in Gruidae showed that the substitution of nucleotide was mainly transitions and transversions,and the ratio of transitions was much larger than transversions.Relationship of several cranes in Gruidae based on Cytochrome-b were estimated using Parsmony analysis and boot strapping.The differences among the sequences of Cytochrome-b from G.joponensis (♂) 、G.vipio (♀) 、G.grus and four interspecific F_1 hybrids between G.joponensis (♂) and G.vipio (♀) were 0.1%～7.2%.The Phylogenetic-tree was made according to Cytochrome-b from several cranes in Gruidae and showed that the phylogenetic relationship between G.joponensis and G.grus was intimate and four filial generations of intercross between G.joponensis (♂) and G.vipio (♀) was relatively close to G.vipio (♀).
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第5期718-723,共6页
Journal of Xiamen University：Natural Science
