过敏性紫癜患儿PBMC表面粘附分子表达、血清抗中性粒细胞胞浆抗体检测情况研究被引量：6

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摘要目的探讨过敏性紫癜(HSP)患儿外周血单个核细胞(PBMC)表面CD54、CD11a、CD11b、CD18表达和血清抗中性粒细胞胞浆抗体(ANCA)阳性率,以及两者在HSP发病中的关系。方法用标记的链霉卵白素-生物素免疫细胞化学染色法检测PBMC表面粘附分子(AMs)CD54、CD11a、CD11b、CD18的表达情况,用间接免疫荧光法(IIF)检测ANCA。结果HSP患儿PBMC表面表达CD54、CD11a、CD11b、CD18的阳性细胞百分率较对照组普遍上调,P<0.001;紫癜性肾炎(HSPN)患儿CD54表达较单纯HSP组升高;ANCA阳性检出率HSP组高于对照组,HSPN组高于单纯HSP无肾脏损害组,差异有显著性,P<0.01;ANCA阳性组的CD54、CD11a、CD11b较ANCA阴性组增高,差异有显著性,P<0.05或<0.01。结论AMs在HSP的发病中发挥一定的作用;HSP患儿血清ANCA阳性率升高,提示ANCA可能参与了HSP的致病过程;HSPN患儿ANCA阳性率明显升高,有助于诊断HSP是否伴肾脏受累;ANCA阳性者AMs高于ANCA阴性者,说明ANCA与AMs在HSP发病过程中可能起协同作用。

作者崔玉霞刘若英李实光杨锡强

机构地区重庆医科大学儿童医院儿科研究所免疫室贵阳医学院附属医院儿科

出处《临床儿科杂志》 CAS CSCD 北大核心 2006年第1期60-62,共3页 Journal of Clinical Pediatrics

关键词过敏性紫癜粘附分子抗中性粒细胞胞浆抗体

分类号 R725.5 [医药卫生—儿科]

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