摘要
目的探讨血红素加氧酶-1(hemeoxygenase_1,HO_1)在小鼠支气管哮喘模型中的抗炎作用。方法用卵清蛋白(OVA)致敏、激发小鼠建立哮喘动物模型,并在致敏、激发过程中分别经Hemin、SnPP处理。分别测定激发后各组动物血清OVA特异性IgE(OVA_SIgE)、支气管肺泡灌洗液(bronchialalveolarlavagefluid,BALF)中细胞总数和嗜酸性粒细胞(eosinophil,EOS),结合病理切片分析气道炎症状况。结果OVA组、Heme组、SnPP组血清OVA_SIgE和BALF中细胞总数及EOS数明显高于正常对照组,但Heme组IgE水平及BALF中细胞总数和EOS数明显低于OVA组;而OVA组和SnPP组间IgE水平及BALF中细胞总数和EOS数差异无显著性;病理切片显示OVA组、Hemin组、SnPP组气道组织均以嗜酸性粒细胞浸润为主,但Hemin组气道炎症仍明显轻于OVA组和SnPP组。结论用Hemin诱导HO_1高表达后血清OVA_SIgE明显下降,气道炎症减轻,提示HO_1在支气管哮喘中具有抗炎作用。
Objective To assess the role of anti-inflammation properties of heme oxygenase-1 （HO-1） in an animal model of asthma. Methods BALB/c mice were sensitized and challenged by ovalbumin （OVA）, and then they were treated with hemin, a HO-1 inducer, and tin-protoporphyrin （SnPP） respectively. Serum OVA specific-IgE was analyzed by ELISA, and the number of total cells and eosinophil （EOS） in bronchial alveolar lavage fluid （BALF） were counted. The histological study was performed to evaluate the airway inflammation. Results Responses to airway inflammation including the level of OVA specific-IgE,the number of total cells and EOS in BALF increased significantly in OVA-sensitized/challenged group, heroin and SnPP group as compared to control group, but hemin group had a lower level of SIg-E, a smaller number of total cells and EOS than that of OVA and SnPP groups. The number of total cells and EOS in BALF of mice sensitized/challenged by OVA increased as compared to that in BALF of control and hemin groups. No significant differences in SIg-E levels, the number of total cells and EOS in BALF were noted between OVA and SnPP groups. Eosinophil infiltration in airway tissues was the main pathological findings in OVA, heroin and SnPP groups, but heroin group had a milder airway response than that of OVA and SnPP groups. Conclusions High-level expression of heroin-induced heine oxygenase-1 could led to a decline of OVA-SIg-E and an alleviation of airway inflammation, suggesting that HO-1 might exert some anti-inflammatory effect and play a role in the pathogenesis of bronchial asthma.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2006年第1期9-12,共4页
Journal of Clinical Pediatrics
基金
由国家自然科学基金资助(NO.30170988)
上海市科委资助(NO.44119662)
上海市教委资助(NO.03BZ04)
