摘要
目的:探讨P物质(SP)在变应性鼻炎(AR)中对RANTES mRNA表达的影响及其意义,深入了解AR的发病机制。方法:以卵清蛋白(OVA)建立AR豚鼠模型(模型组)。然后用SP滴鼻激发AR各组,并与正常豚鼠对照(对照组),观察其症状和鼻黏膜病理学变化,并对鼻腔灌洗液中的嗜酸粒细胞进行计数。采用RT-PCR方法,对各组动物鼻黏膜组织中RANTES mRNA的表达水平进行相对定量比较。结果:SP激发能诱发正常的豚鼠出现相似的AR症状,并能加重模型组豚鼠AR症状和鼻黏膜炎症。而且SP滴鼻使AR豚鼠鼻黏膜RANTES mRNA的表达显著增高(P<0.05),鼻灌洗液中嗜酸粒细胞数亦明显增高(P<0.01)。结论:在AR的发病机制中,SP能诱导鼻黏膜表达RANTES,促进嗜酸粒细胞趋化与转运,引发和加重AR的炎症反应。
Objective：To study the significance and effect of SP on the expression of RANTES mRNA in allergic rhinitis （AR） , and deeply understand the pathogenesis of AR. Method：The model of AR was established in randomly selected healthy guinea pigs by using ovalbumin. Then the nasal dripping of SP was used to challenge the AR groups, as compared with the normal controls, in observation of the symptoms and histopathoiogic changes in the nasal mucosa. The number of eosinophil in the nasal lavage fluid was also counted. By means of RT-PCR, the expressions of RANTES mRNA in the nasal mucosa of the different groups were quantitatively compared. Result： SP challenge induced similar AR symptoms in the normal group and aggravated AR symptoms and inflammation in the nasal mucosa in the model of AR groups. The expression level of RANTES mRNA （ P 〈0.05） and the number of eosinophil （ P 〈0. 01） in AR groups increased significantly after challenged with SP. Conclusion： In the pathogenesis of AR, SP induces the expression of chemokine RANTES in nasal mucosa, promotes eoslnophil chemotaxis and migration, induces and aggravates the allergic inflammatory reaction in AR.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2006年第2期73-77,共5页
Journal of Clinical Otorhinolaryngology
基金
国家自然科学基金资助项目(No:30271406)
上海市卫生系统百名跨世纪优秀学科带头人培养基金资助项目(No:98BR003)
