摘要
报道了用酶提取侧柏叶中的挥发性组分,考察pH值、酶用量、样品粒度等因素对提取率的影响,测得其挥发油的含量为6.0%(w)。采用GC-MS法分离确认出38种化学成分,其中主要成分为雪松烯,占总挥发油的41.25%。并将酶提取(CE)法与同时蒸馏萃取(SDE)法进行了对比。
The cellulase extraction of volatile constituents in leaf twigs of Biota Orientalis was reported. The effects of pH, the amount of cellulase and the fineness number of the sample were discussed. The average yield obtained for volatilizing oil was 6.0% （w）. Thirty eight chemical compoents in the volatilizing oil were identified by GC - MS method, It was found that cedrene was the major constituent amounting to 41.25%. The relative contents of the chemical components and the total ion chromatogram obtained by cellulase extraction （CE） method were compared with those obtained by simultaneous distillation extraction （ SDE ） method.
出处
《分析测试学报》
CAS
CSCD
北大核心
2006年第1期34-37,共4页
Journal of Instrumental Analysis
基金
辽宁省教育厅科学技术基金资助课题(20331079)
关键词
酶提取法
侧柏叶
挥发性成分
同时蒸馏-萃取法
雪松烯
Cellulase extraction （ CE ） method
Leaf twigs in Biota Orientalis
Volatile constituents
Simultaneous distillation extraction（SDE） method
Cedrene
