摘要
目的检测类风湿关节炎患者(RA)血清中的环瓜氨酸肽抗体,探讨其在RA诊断中的价值。方法用ELISA方法分别检测228例RA、192例非RA和71例正常人中抗环瓜氨酸肽(CCP)抗体;用间接免疫荧光法和速率散射比浊法检测抗角蛋白抗体(AKA)和类风湿因子(RF),分析CCP抗体的水平及与AKA、RF的相关性。结果CCP抗体的阳性率在RA中为85.53%,在非RA中为5.21%,正常人全部阴性。3种抗体对RA诊断的敏感性和特异性分别为CCP85.53%、96.20%,AKA59.65%、94.68,RF81.14%、79.47%。CCP抗体与AKA在RA患者血清中的阳性率之间差异非常显著,与RF差异不显著。3种方法的检测结果间存在相关性。结论用ELISA法检测血清中CCP抗体简便、结果可靠,对RA诊断具有高度的敏感性和特异性。
Objective To detect anti- cyclic citrullianted peptide （CCP） antibody in patents with rheumatoid arthritis （RA） and explore its diagnostic value.Methods Anti - CCP antibodies were examined by an ELISA from 228 RA, 192 non -RA patients and 71 normal donors sera. Anti- keratin antibody （AKA） were detected by indirect immunofluorescence （IIF）. Rheumatoid factor（RF） were assayed by rate nephelometry method. Results were analysed by comparing the correlations among the anti- CCP,AKA and RF. Results Among 228 patients with RA, 195 patients showed positive of anti - CCP（85.53% ）. Whereas,the positive percent was only 5.21 % in 192 non- RA patients. The sensitivity and specificity of anti- CCP,AKA and RF were 85.53%and 96.20% ;59.65%,94.68% ;81.14%,79.47%in RA respectively. The data showed that there was a significant difference between the two methods of anti - CCP and AKA assaying. However, no difference was found between anti - CCP and RF assaying. Anti- CCP was associated with AKA and RF.Conelusion Detecting anti - ccp by ELISA may be a brief,standard and reliable method. It might present more sensitive and specific in clinical diagnosis on RA.
出处
《中国实验诊断学》
2006年第1期84-87,共4页
Chinese Journal of Laboratory Diagnosis
基金
2004年河北省科技攻关项目(编号042761324)
关键词
类风湿性关节炎
CCP抗体
AKA
RF
Rheumatoid arthritis
Anti- cyclic eitrullinated peptide antibody
anti - keration antibody
rheumatoid factoor
