期刊文献+

重金属离子对凡纳滨对虾肝胰脏MT含量的影响 被引量:27

Effects of heavy metal ions on metallothionein concentration in hepatopancreas of Litopenaeus vannamei
在线阅读 下载PDF
导出
摘要 The metallothionein(MT) concentration in hepatopancreas of Litopenaeus vannamei changed significantly with the increasing of metal ions concentration and prolongation of exposure time(P<0.05),whereas that in the control groups had no marked changes(P>0.05).Exposed to three heavy metal ions,the MT concentration in hepatopancreas changed very significantly in 6 h(P<0.01). The MT concentration inducted by Cu2+(0.1,0.2,0.5,1 mg·L-1), Zn2+(1,2, 5 mg·L-1)and Cd2+(0.05,0.1 mg·L-1)increased in 96 h with time and changed stably after 48 h.In addition,the MT concentration inducted by Zn2+(10 mg·L-1) and Cd2+(0.25,0.5 mg·L-1)was up to max.at 48 h,began to decrease with time passing by,and still higher than that in the control groups.Meanwhile the inductive ratio of three heavy metal ions on metallothionein concentration were Cd2+>Cu2+>Zn2+ in experimental time. The metallothionein (MT) concentration in hepatopancreas of Litopenaeus vannamei changed significantly with the increasing of metal ions concentration and prolongation of exposure time ( P 〈 0.05 ), whereas that in the control groups had no marked changes ( P 〉 0.05 ). Exposed to three heavy metal ions, the MT concentration in hepatopancreas changed very significantly in 6 h (P〈0.01). The MTconcentration inducted by Cu^2+(0.1, 0.2, 0.5, 1 mg·L^-1), Zn^2+(1, 2, 5 mg·L^-1) and Cd^2+ (0.05, 0.1 mg·L^-1 ) increased in 96 h with time and changed stably after 48 h. In addition, the MT concentration inducted by Zn^2+ ( 10 mg·L^-1 ) and Cd^2+ (0.25, 0.5 mg·L^-1) was up to max. at 48 h, began to decrease with time passing by, and still higher than that in the control groups. Meanwhile the inductive ratio of three heavy metal ions on metallothionein concentration were Cd^2+ 〉Cu^2+ 〉Zn^2+ in experimental time.
出处 《水产学报》 CAS CSCD 北大核心 2005年第5期715-718,共4页 Journal of Fisheries of China
基金 山东省科技兴海项目<低盐度地下卤水凡纳滨对虾养殖技术的开发研究>资助(2001-3-6)
关键词 重金属离子 凡纳滨对虾 肝胰脏 金属硫蛋白 heavy metal ions Litopenaeus vannamei hepatopancreas metallothionein(MT)
  • 相关文献

参考文献16

  • 1Kagi J H R, Schaffer A. Biochemistry of metallothionein[J].Biochemistry,1988,27:8509-8515.
  • 2茹炳根,潘爱华,黄秉乾,张建业.金属硫蛋白[J].生物化学与生物物理进展,1991,18(4):254-259. 被引量:89
  • 3刘安玲,朱必凤.金属硫蛋白的研究进展[J].韶关学院学报,2001,22(3):86-91. 被引量:23
  • 4牛长缨,姜勇,雷朝亮,胡萃.无脊椎动物金属硫蛋白的研究[J].动物学杂志,2002,37(1):72-76. 被引量:20
  • 5叶寒青,杨祥良,周井炎,徐辉碧.环境污染物镉毒性作用机理研究进展[J].广东微量元素科学,2001,8(3):9-12. 被引量:41
  • 6Pedersen S N, Lundebye A K, Depledge M H. Field application of metallonthionein and stress protein biomarkers in the shore crab (Carcinus maenas) exposed to trace metals[J].Aquatic Toxicology,1997,37:183-200.
  • 7Martinez M,Jose D R,Torreblanca A,et al. Effects of cadmium exposure on zinc levels in the brine shrimp Artemia parthenogenetica[J]. Aquac,1999,172:315-325.
  • 8Olafson R W, Kearns A, Sim R G. Heavy metal induction of metallothioneion synthesis in the hepatopancreas of the crab Scylla serrata[J]. Comp Biochem Physiol,1979,62:417-424.
  • 9Scheuhammer A M,Cherian M G. Quantification of metallothioneins by a silver-saturation method[J].Toxicol Appl Pharmacol,1986,82,417-425.
  • 10Correia A D, David R L,Costa M H. Effects of water-borne copper on metallothionein and lipid peroxidation in the marine amphipod Gammarus locusta[J]. Marine Environmental Research,2002,54:357-360.

二级参考文献50

  • 1张亨山.金属硫蛋白的抗过氧化作用[J].国外医学(卫生学分册),1994,21(2):68-71. 被引量:7
  • 2周杰昊,程时.金属硫蛋白与医学[J].生理科学进展,1995,26(1):29-34. 被引量:29
  • 3李令媛,马宏宝,吕迎春,茹炳根.金属诱导条件下刺猬各组织器官金属硫蛋白含量的比较分析[J].兽类学报,1995,15(1):65-70. 被引量:7
  • 4林稚兰,常立梅.真核微生物的类金属硫蛋白[J].生物工程进展,1996,16(3):33-43. 被引量:15
  • 5[1]Margoshes M, Vallee B L. A cadmium binding protein from equine renal cortex. J Am ChemSoc, 1957,79:4813.
  • 6[2]Hamer D H. Metallothionein. Ann Rev Biochem,1986,55:913~951.
  • 7[3]Silar P, Theodore L,Mokdad R et al. Metallothionein Mto gene of Drosophila melanogaster: structure and regulation. J Mol Biol, 1990,215:217~224.
  • 8[4]Frazier J M, George S G, Overnell J et al. Characterization of two molecular weight classes of cadmium binding proteins from the mussels, Mytilus edulis. Comp Biochem Physiol,1985,80C:257~266.
  • 9[5]Mackay E A, Overnel J, Dunbar B et al. Complete amino acid sequences of five dimeric and four monomeric forms of metallothionein from the edible mussel Mytilus edulis. Eur J Biochem, 1993,218:183~194.
  • 10[6]Carpene E, Cattani O, Hakim G et al. Metallothionein from foot and posterior adductor muscle of Mytilus galloprovincialis. Comp Biochem Physiol, 1983,74C:331~336.

共引文献246

同被引文献460

引证文献27

二级引证文献224

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部