摘要
目的:采用RP-HPLC-ELSD法建立知母药材的指纹图谱。方法:以Krom asil C18(250.0 mm×4.6 mm,5μm)为色谱柱,采用甲醇-水进行梯度洗脱,流速1.0 m l.m in-1,柱温30℃,检测器温度100℃,气体流速2.0 m l.m in-1。结果:共标出知母药材苷元类成分8个共有峰,重复性、精密度和溶液稳定性试验中,峰面积大于10%总蜂面积的共有峰,其相对峰面积的RSD值均小于3%,符合《中药注射剂指纹图谱研究的技术要求(暂行)》中的有关规定。结论:RP-HPLC-ELSD法稳定、可靠、重复性好,为知母药材质量控制提供参考。
Objective To establish the fingerprint chromatogram of crude Anemarrhena asphodeloides, Methods HPLC with Kromasil C 18 （250.0mm × 4.6mm,5μm） column was used, the CH3OH-H20 （gradient elution） as a mobile phase and with the column temperature of 30 ℃. The ELSD detector temperature was 100℃ and the gas flow-rate was 2.0 ml·min^-1. Results 8 peaks were separated respectively on the HPLC fingerprint. The RSD of precision, reproducibility and stability of the solution were less than 3%. Conclusion The method is simple and accurate with a good reproducibility and can be used for a quality control method for Anemarrhena asphodeloides.
出处
《东南大学学报（医学版）》
CAS
2006年第1期24-27,共4页
Journal of Southeast University(Medical Science Edition)
关键词
知母/化学
皂苷类/分析
色谱法
高压液相
指纹图谱
ANEMARRHENA ASPHODELOIDES/chemistry
saponins/analysis
chromatography, high pressure liquid
fingerprint
