摘要
人食管癌上皮细胞系Ｅｃａ１０９可以表达癌胚基因产物胎盘型碱性磷酸酶（ＰＬＡＰ）．并且ＰＬＡＰ活性在强的松龙诱导下升高。蛋白质合成抑制剂环己亚胺在强的松龙作用的初始阶段及强的松龙致ＰＬＡＰ活性快速升高阶段时加入，均表现对强的松龙效应的抑制。而ＲＮＡ合成抑制剂放线菌素Ｄ与强的松龙同时作用于细胞时，则抑制强的松龙的诱导效应，但在强的松龙作用后ＰＬＡＰ活性快速升高阶段加入放线菌素Ｄ时，至少在２４小时内不产生对强的松龙效应的抑制。结果提示．强的松龙对ＰＬＡＰ的诱导作用与新的ＲＮＡ及蛋白质合成有关。强的松龙还可引起Ｅｃａ１０９细胞生长增殖减慢。
The human esophageal epithelial cell line Eca 109 cells were found to express the oncoplacental gene product,placental alkaline phosphatase(PLAP).The effect of prednisolone on the induction of PLAP in Eca 109 cells was studied.The results showed that prednisolone could cause dramatic increase in the activity of PLAP.The inhibitor of protein biosynthesis, cycloheximide,blocked the induction of PLAP even when added during rapid increasing phase of PLAP.The inhibitor of RNA biosynthesis,actinomycin D.blocked the induction when added at the same time as prednisolone.While added during the rapid increasing Phase of PLAP activity,actinomycin D had no effect within 24h after treatment.The results suggested that the new RNA and protein synthesis may be involved in prednisolone induction of PLAP. Prednisolone could also inhibit the proliferation of Eca 109 cells.
出处
《河北医科大学学报》
CAS
1996年第2期65-67,共3页
Journal of Hebei Medical University
关键词
ECA109细胞
胎盘
碱性磷酸酶
强的松龙
诱导作用
Eca 109 cell
placental alkaline phosphatase
prednisolone
induction
cycloheximide
actinomycin D
