摘要
介绍了鸟枪法蛋白质组的系统组成,研究进展以及在医药领域的应用情况．鸟枪法蛋白质组学自出现以来 已经成为研究复杂蛋白质混合物的有力工具．随着色谱和质谱技术的提高,样品制备方法的改进以及多向色谱和蛋 白质组信息学的不断发展,鸟枪法蛋白质组学也遇到了机遇和挑战．
This review introduces the components of this proteomics strategy and describes selected recent applications and emerging innovations in the evolution of shotgun proteomics. Shotgun proteomics has emerged as a powerful approach for the analysis of complex protein mixtures, including biofluids, tissues, cells, organelles or protein complexes. Having evolved from the integration of chromatography and mass spectrometry, innovations in sample preparation, multidimensional chroT matography, mass spectrometry and proteomic informatics continually facilitate, enable and challenge shotgun proteomics. As a result, shotgun proteomics continues to evolve and enable new areas of biological research, and is beginning to impact human disease diagnosis and therapeutic intervention.
出处
《阜阳师范学院学报（自然科学版）》
2005年第4期41-45,共5页
Journal of Fuyang Normal University(Natural Science)
基金
国家973课题(2006CB100100)
关键词
鸟枪法
蛋白质组学
研究进展
应用
shotgun
proteomics
research innovation
recent applications
