摘要
目的研究不同浓度胰升糖素样肽-1(GLP-1)对大鼠胰岛β细胞胰岛素基因表达的影响,探讨GLP-1在2型糖尿病治疗中的作用。方法不同刺激浓度的GLP-1(0 nmol/L、1 nmol/L、10 nmol/L、102nmol/L、103nmol/L)与葡萄糖浓度分别为2.2 mmol/L、5.5 mmol/L、11.1 mmol/L的RPMI1640营养液共培养24 h后,提取细胞总RNA,运用半定量RT-PCR技术检测胰岛素基因表达的变化。结果葡萄糖浓度为2.2 mmol/L和5.5 mmol/L时,随GLP-1浓度升高,胰岛素基因表达量呈钟型方式升高;葡萄糖浓度为11.1 mmol/L时,随GLP-1浓度升高,胰岛素基因表达量呈浓度依赖性升高。结论GLP-1以葡萄糖依赖和浓度依赖方式促进胰岛素基因的表达。
Objective To investigate the effect of different concentrations of glucagon-like peptide-l（GLP-1） on insulin gene expression in i^olated rat islets, and study the therapeutic roles of GLP-1 in type 2 diabetes mellitus. Methods GLP1 was added at final concentrations of 1, 10, 10^2 and 10^3 nmol/L to media containing 2.2 mmol/L （low）, 5. 5 mmol/L （normal）, 11.1 mmol/L （high） glucose. All islets samples were harvested after 24 h, ,and total RNA was extracted in a single series for RT PCR. Results At low and normal glucose concentrations, GLP-1 increased insulin mRNA level in a bell-shaped fashion. At high glucose concentrations, a dosedependent increase in insulin mRNA levels was observed. Conclusion Insulin mRNA level is upregulated by GLP-1 dose dependently and glucose-dependently.
出处
《江苏医药》
CAS
CSCD
北大核心
2006年第1期45-46,共2页
Jiangsu Medical Journal
基金
江苏省医学重点基金资助项目(2001031)
