摘要
本试验研究了用根癌土壤杆菌转化马铃薯的各种条件，在采用品种东农３０３的材料和Ａ２０８、Ａ２８１两种野生型根癌土壤杆菌的研究中筛选出了各个优化转化条件，建立了优化遗传转化系统．其结果为①外植体：块茎；②根癌土壤杆菌浓度：５×１０８ＣＦＵ／ｍｌ；③ｐＨ值（共培养）：５．８；④共培养中加入乙酰丁香酮浓度：４０μｍ；⑤初始共培养时间：２天等的效果最佳．
Various conditions for potato (Solanum tubrosum L.) transformation with Agrobacterium tumefaciens A208 and A281 and cv. Dong Nong 303 were studied to construct optimized transformation system. The results were as follovos: ① explant, tuber; ② density of Agrobacterium tumefaciens, 5×108 CFU / ml; ③ pH value (co-cultivation), 5.8; ④ the concentration of added acctosyringonc, 40 μM; ⑤ co-cultivation time, 2 days.
出处
《马铃薯杂志》
1996年第2期65-69,共5页
Chinese Potato Journal
关键词
马铃薯
基因转移
转化系统
potato, gene tranfer, Agrobacterium tumefaciens, transformation system
