摘要
目的研究在四氧嘧啶诱导小鼠糖尿病发病过程中维生素D受体的作用。方法采用维生素D受体基因敲除小鼠研究维生素D受体在小鼠糖尿病发病过程中的作用。四氧嘧啶尾静脉注射诱导小鼠糖尿病,PCR法鉴定基因型,自动生化分析仪测定血钙,血糖仪法测定血糖,试纸法尿糖定性。结果维生素D受体基因敲除小鼠和野生型小鼠血钙水平无显著差别;维生素D受体敲除小鼠血糖和糖尿病发病率高于同窝野生型(P<0.05)。结论维生素D受体基因敲除增加了小鼠糖尿病的发病率,但其详细机理需要进一步研究。
Objective To study the effects of vitamin D receptor on Alloxan-induced diabetes in mice. Methods Experimental mice diabetes model were produced by intravenous injection of Alloxan. There were two kinds of mice in this study, which were vitamin D receptor knockout mice （VDR-/-） and wild type mice（WT） in the same litter. The effects of VDR on diabetes mice were investigated by comparing the difference in serum glucose, urine glucose and incidence of diabetes between the two kinds of mice. Results There was higher level of serum glucose and incidence of diabetes in VDR-knockout mice. Conclusion VDR has a significantly protective effect on alloxan-induced diabetes in mice.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2005年第6期966-968,共3页
Suzhou University Journal of Medical Science
基金
苏州大学医学发展基金资助(EE126509)
