摘要
目的:研究乳腺丝氨酸蛋白酶抑制剂基因(marray serine proteinase inhibitor,Maspin)、血管内皮生长因子(vascular endothelial growth factor,VEGF)在口腔鳞癌中的表达及其相关性。方法:应用RT-PCR方法,分别对18例口腔鳞癌的癌组织、癌旁组织及相应正常组织标本行Maspin和VEGFmRNA含量的半定量测定,应用随机区组设计的F检验和Person直线相关分析进行统计分析。结果:MaspinmRNA在口腔鳞癌的癌组织、癌旁组织和正常组织中表达逐渐增加(P<0.01),且三者之间表达差异均有显著性(P<0.05);VEGFmRNA在癌组织、癌旁组织、正常组织中的表达依次降低(P<0.01),且癌组织和正常组织、癌组织和癌旁组织之间表达差异有显著性(P<0.05);口腔鳞癌中,Maspin和VEGFmRNA表达呈负相关(P<0.01);Maspin和VEGF基因mRNA表达与肿瘤淋巴结转移相关(P<0.05),与肿瘤的临床分期和病理分级无关。结论:Maspin基因在口腔鳞癌的发生发展过程中可能起着重要抑癌作用;Maspin可能通过下调VEGF来发挥抑癌作用。
PURPOSE： To study the expression of Maspin （marray serine proteinase inhibitor） and VEGF（vascular endothelial growth factor） gene in oral squamous cell cancers （OSCC） and the relationship between Maspin and VEGF. METHODS： mRNA expression of Maspin and VEGF gene was investigated by RT-PCR in every samples of 18 OSCC cases. F test and Person test were used for data analysis. RESULTS： mRNA expression of Maspin gene increased gradually in OSCC, paratumor and normal epithelium （P〈0.01）; mRNA expression of VEGF gene decreased gradually in OSCC,paratumor and normal epithelium （P〈0.01）; The expression of Maspin and VEGF gene was negatively correlated in OSCC（P〈0.01） ; The expression of Maspin gene was negatively associated but VEGF gene was positively associated with lymph node metastasis in OSCC （P〈0.05）; The expression of Maspin and VEGF gene had no relation with the histological grade or tumor size in OSCC. CONCLUSION： Maspin and VEGF gene may play an important role in the development of OSCC and Maspin gene may down-regulate the expression of VEGF gene.
出处
《上海口腔医学》
CAS
CSCD
2005年第6期557-560,共4页
Shanghai Journal of Stomatology
基金
湖南省自然科学基金(03JJY3045)
