摘要
目的 研究类金刚石（diamond—like carbon，DLC）镀膜形状记忆合金对体外培养的成骨细胞生物学行为的影响。方法 选择规格相同的DLC镀膜镍钛（NiTi）记忆合金（镀膜组）、非镀膜记忆合金（非镀膜组）各30片与体外培养的成骨细胞混合培养，并设立空白对照组（不加材料的单纯细胞培养组），观察细胞的增殖情况。收集第1～5天的细胞培养液，测定其中的碱性磷酸酶（alkaline phosphatase，ALP）、Ni^2＋含量及成骨细胞计数。结果 镀膜组和对照组细胞计数及ALP浓度均高于非镀膜组；细胞计数，第3、4及5天，镀膜组及对照组与非镀膜组比较，差异有统计学意义（P〈0．05）；ALP浓度，镀膜组第2、3及5天，对照组第3、4及5天，与非镀膜组比较，差异有统计学意义（P〈0．05）。Ni^2＋含量低于非镀膜组，第3、4及5天与非镀膜组比较，差异有统计学意义（P〈0．05）。结论 DLC镀膜形状记忆合金的组织相容性明显优于非镀膜组。
Objective To investigate the biocompatibility of diamond-like carbon （DLC） coated Nickel-Titanium shape memory alloy with osteoblasts cultured in vitro. Methods Rabbit＇s osteoblasts were incubated with DLC-coated Nickel-Titanium shape memory alloy disks and uncoated ones of equal size for 5 days. The control group （without shape memory alloy in culture media） was performed simultaneously. The cultured cells were counted and graphed. The samples from culture media were collected and the.concentrations of alkaline phosphatase （ALP） and nickel（Ni^2＋） were measured from the 1st to 5th day respectively. Results The proliferation of osteoblasts and the concentration of ALP in both DLC-coated group and control gruop was higher than uncoated group. The proliferation of osteoblasts on the 3rd, 4tb, and 5tb day in both DLC-coated group and control group was significantly higher than that in the uncoated group（P〈0.05）. The concentration of ALP in DLC-coated group on the 2nd, 3rd, and 5th day and in the control group on the 3rd, 4th, and 5tb day was significantly higher than that in the uncoated group（P〈0.05）. The concentration of Ni^2＋ on the 3rd, 4tb, and 5th day was significantly lower than that in the uncoated group（P〈0.05）. Conclusion DLC-coated Nickel-Titanium shape memory alloys appears to have better biocompatibility with osteoblast cultured in vitro compared to uncoated ones.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2006年第1期5-8,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
类金刚石
镍钛记忆合金
镀膜
成骨细胞
生物相容性
Diamond-like carbon Nickel-Titanium shape memory alloy The coated Osteoblast Biocompatibility
