摘要
目的观察骨延长区骨组织修复过程中胶原变化特点及规律。方法健康新西兰白兔27只,制成左胫骨中段骨延长模型,于术后第5天以1.5mm/d延长,共延长14d。于延长结束时,延长结束后7、14、21、30、40、50、60和70d分批处死动物,取材(n=3)。石蜡包埋切片,饱和苦味酸天狼星红染色,偏光显微镜观察新骨组织的胶原变化。结果延长区胶原变化主要有以下特点:1在延长结束时及延长结束后的早期纤维组织中,除型胶原外,还含有大量型胶原;2在软骨组织中有、和型胶原;3假性生长板中存在、和型胶原;4骨延长区新骨再生与修复的后期型胶原占绝对优势。结论骨延长区的骨组织修复是在牵张应力下进行的,其胶原变化显示出与骨折愈合不同的特点;饱和苦味酸天狼星红染色后,在偏光显微镜下可清晰地区分、和型胶原,为研究骨组织修复过程中胶原变化的分型提供了新的方法。
Objective To investigate the feature and regularity of the collagen change in bone healing during bone lengthening. Methods Bone lengthening model was made in the middle segment of the rabbit tibia. Five days after the model was established, the bone was lengthened 1.5 mm per day for 14 days. The rabbits were put to death after elongation, 7,14,21,30,40,50,60 and 70 days after elongation. The distracted area of the bone was imbedded with paraffin. After being stained by the pierie acid-sirius red staining, the slice was observed under polarized microscope. Results The features of the collagen change in the distracted bone were as follows： ① In the fibrous tissue of the distracted area during lengthening period and the early stage after lengthening, there was not only collagen Ⅰ but also much collagen Ⅰ . ② Collagen Ⅰ , Ⅱ and Ⅲ were observed in the cartilage. ③ Collagen Ⅰ ,Ⅱ and Ⅲ were also observed in the pseudo-growth plate. ④ Collagen Ⅰ took the dominance during lengthening period and the late stage after lengthening. Conclusion New bone formation in bone lengthening is under the distracted force, so the collagen changes have different features compared with that in fracture healing. Collagen Ⅰ, Ⅱ and Ⅲ can be identified by picric acid-sirius red staining and polarized microscope, so a new method for studying the collagen typing in bone repairing is provided.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2006年第1期1-4,共4页
Chinese Journal of Reparative and Reconstructive Surgery
基金
云南省应用基础研究基金资助项目(98C109M)~~
关键词
骨延长
胶原
天狼星红染色
偏光显微镜
Bone lengthening Collagen Sirius red staining Polarized microscope
