摘要
背景:淫羊藿具有促进成骨细胞增殖分化的作用。目的:观察磷酸钙骨水泥与淫羊藿复合材料对骨质疏松大鼠骨折愈合的影响。方法:将SD大鼠随机分组:实验组切除双侧卵巢建立骨质疏松动物模型,对照组仅切除卵巢周围少许脂肪组织。实验组将造模成功动物制作右侧股骨远端松质骨骨折,随机分3组治疗:克氏针内固定组、磷酸钙骨水泥组、磷酸钙骨水泥-淫羊藿复合物组。结果与结论:①碱性磷酸酶活性:治疗后2,4,8周时,3组均有不同程度升高,磷酸钙骨水泥-淫羊藿复合物组最高(P<0.05)。12周时,磷酸钙骨水泥-淫羊藿复合物组基本恢复至正常范围,其他两组虽有所下降,但仍维持在一个相对较高水平。②生物力学检测:磷酸钙骨水泥-淫羊藿复合物组骨折段承受的最大载荷明显高于其他两组(P<0.05),且增加幅度最大。③组织学检查:磷酸钙骨水泥-淫羊藿复合物组纤维骨痂及骨样骨痂出现较其他两组早,且数量较多,骨愈合质量明显高于其他组。表明磷酸钙骨水泥-淫羊藿复合材料具有较好的骨诱导活性,可有效促进骨质疏松大鼠骨折的愈合。
BACKGROUND： Epimedium can promote the proliferation and differentiation of osteoblasts. OBJECTIVE： To observe the effects of calcium phosphate cement （CPC）/epimedium composite materials on fracture healing of osteoporotic rats. METHODS： Sprague Dawley rats were randomly divided into control group and experimental group. Ovariectomy-induced osteoporosis rat models were established in the experimental group, while surgical excision of a small amount of fatty tissue around the ovaries was performed in the control group. Cancellous bone fracture was prepared in the distal end of the right femur of rats in the experimental group, and the rats were subdivided into three groups for treatment： Kirschner wire group, CPC group and CPC/epimedium group. RESULTS AND CONCLUSION： Alkaline phosphatase activity： increasing in varying degrees of alkaline phosphatase activity could be seen in the three groups at 2, 4 and 8 weeks after treatment, and the improvement in alkaline phosphatase activity of the CPC/epimedium group was the highest （P 〈 0.05）. At 12 weeks, alkaline phosphatase activity of the CPC/epimedium group returned to normal, and those of the other two groups maintained a high level despite some decrease. Biomechanical detection： the maximum load of the CPC/epimedium group was obviously higher than those of the two other groups （P 〈 0.05）, and the rate of increase in the maximum load of the CPC/epimedium group was the highest. Histological examination： fibrous osteotylus and bone callus of the CPC/epimedium group appeared more early, and both the quantity and the quality of new bone were superior to those of the other groups. It is indicated that the CPC/epimeclium artificial bone possesses a good osteoinductive activity, and can improve the fracture healing of osteoporotic rats.
出处
《中国组织工程研究》
CAS
CSCD
2012年第29期5389-5395,共7页
Chinese Journal of Tissue Engineering Research
基金
南京市医学科技发展资金资助项目(YKK08126),项目名称:淫羊藿缓释体系对骨质疏松型骨折疗效研究~~
