摘要
目的观察组织蛋白酶B(cathepsinB,CB)和L(cathepsinL,CL)在人早期妊娠绒毛及蜕膜中的表达,探讨它们在胚胎着床及发育过程中的作用。方法研究组包括:正常早期妊娠行人工流产者30例、早期自然流产者25例、正常分泌期子宫内膜10例和葡萄胎15例。应用免疫组织化学法检测CB和CL的表达。结果CB、CL主要阳性表达于正常早期妊娠滋养层细胞和蜕膜细胞。CB在正常分泌期内膜、正常早期妊娠蜕膜、自然流产蜕膜中的阳性率表达分别为10%(1/10)、83.3%(25/30)、32.0%(8/25);CL则分别为0、63.3%(19/30)、32.0%(8/25);CB和CL在3组间的表达有显著性差异(P<0.05)。CB在早期妊娠绒毛、自然流产绒毛、葡萄胎组织中表达强阳性率分别为10.0%(3/30)、0、66.7%(10/15);CL则分别为26.7%(8/30)、4.0%(1/25)、80.0%(12/15)。CB和CL在3组间的表达有显著性差异(P<0.05)。结论CB和CL在正常和异常妊娠蜕膜和绒毛中的表达差异提示它们胚胎着床过程中可能起着很重要的作用。
Abstract： Objective To study the expression of cathepsins B and L in first-trimester gestational decidua and chorionic villi. Methods The decidua and chorionic villi in the first trimester of gestation were obtained from 30 women undergoing induced abortion, 25 with spontaneous abortion, l0 with normal endometrium in secretory phase, and 15 with bydatidiform mole in whom the expression of cathepsins B and L was determined by immunohistochemistry. Results Positive staining for cathep- sins B and L were mainly detected in the trophoblasts and decidual cells. The positive expression rate ofcathepsin B in the normal endometrium of secretory phase, dec idua in the first trimester of gestation and spontaneous abortion were 10% （1/10）, 83.3% （25/30）, and 32.0% （8/25）, respectively, and the rate of cathepsin L expression in the endometrium in secretory phase, decidua of induced abortion and spontaneous abortion were 0.0%, 63.3% （19/30）, and 32.0% （8/25） respectively, showing significant difference in the expression rates of cathepsins B and L between the 3 groups （P〈0.05）. Strong positive expression of cathepsin B in chorionic villi of induced abortion, spontaneous abortion and hydatidiform mole were detected at the rates of 10.0% （3/30）, 0.0%, and 66.7% （10/15）, respectively, and cathepsin L at the rates of 26.7% （8/30）, 4.0% （1/25）, and 80.0% （12/15）, respectively, with significant difference in strong cathepsins B and L expressions between the 3 groups （P〈0.05）. Conclusion The expression of cathepsins B and L differs in normal and abnormal deciduas and chorionic villi of early pregnancy, suggesting that the cathepsins may play important roles in the process of implantation.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第11期1365-1368,共4页
Journal of First Military Medical University
基金
973国家重点基础研究发展规划项目(G1999055093)~~
