摘要
目的通过碱性成纤维细胞生长因子(bFGF)调节卵磷脂和多聚赖氨酸共同包埋的聚乳酸三维支架与兔关节软骨细胞的体外培养,观察bFGF对组织工程软骨细胞生长的调节作用,探寻软骨组织工程的适宜方法。方法将体外培养的第3代兔关节软骨细胞种植于卵磷脂和多聚赖氨酸包埋的聚乳酸三维支架,加入bFGF共同培养,行大体、倒置显微镜、扫描电镜及免疫组织化学观察,分析软骨组织的形成,并行统计学分析。结果通过bFGF调节的卵磷脂和多聚赖氨酸共同包埋的聚乳酸三维支架—关节软骨细胞复合物在培养过程中不仅能够保持其初始外形,而且能保持种植细胞稳定的三维均相分布,无细胞脱落现象。同时脆性亦逐渐降低,韧性增加,有弹性,表面湿润、光滑,培养2周后,逐渐形成富含Ⅱ型胶原,具有典型软骨组织结构的成熟工程化软骨。其细胞生长及胶原分泌量明显强于对照组,统计学分析有显著性差异。结论bFGF能够促进组织工程软骨细胞的增殖,并具有增强软骨细胞功能的作用。
Objective Culturing rabbit articular chondrocytes in vitro and seeding on polylactic acid （ PLA ） coated with lecithin and pnly-l-lysine modulated by bFGF to find a suitable method for cartilaginous tissue engineering Methods The articular chondrocytes were isolated enzymatically from the articular cartilage of young rabbits, and cultured in vitro. Collecting the chondrocytes of the third passage and seeding on three-dimentional scaffold of polylactic acid coated with lecithin and poly-l-lysine. At the same time, basic fibroblast growth factor （ bFGF ） was added. Proceeing series of detections when the cell-scaffold complexes were cultured more than two weeks, such as macroscope, invert microscope, light microscope, scanning electron microscope and immunohistochemistry of collagen Ⅱ . Results The cell-scaffold complexes modulate by bFGF could not only keep their original shapes, but also maintain the stable homogeneous three-dimensional distribution of chondrocytes without cell falling during the cultivation. At the same time, the complexes were gradually decreasing the consistency, however, increasing the ductility with elasticity and lubrication surface. After the second week, the complexes were gradually reorganized into the mature engineered cartilage with typical cartilaginous histological structure with rich collagen Ⅱ . Conclusion bFGF can facilitate the regeneration and maturation of tissue-engineered articular cartilage.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2005年第24期1590-1593,共4页
Chinese Journal of Surgery
