摘要
目的:探讨肢体缺血再灌注(limbs ischemia-reperfusion,LIR)对脑组织的影响。方法:复制大鼠LIR模型,采用尼氏染色观察脑组织病理学改变,TUNEL法检测细胞凋亡情况,免疫组化和Western印迹法检测凋亡相关因子Bcl-2、细胞色素C(cyto C)、Caspase-3的表达变化。结果:大鼠LIR后,脑组织海马区、中脑红核区神经元细胞受损,脑组织出现水肿裂隙,cyto C、 Caspase-3凋亡相关蛋白表达明显上调,24 h达高峰．与细胞凋亡数量的增加相一致,Bcl-2表达与再灌时程呈负相关。结论:LIR 后可导致脑组织损伤,并出现细胞凋亡,其发生机制与Caspase-3、cyto C、Bcl-2凋亡相关蛋白的表达变化有关。
Objective： To evaluate the effects of hind limbs ischemia-reperfusion （LIR） on brain injury, Methods： Limbs ischemia- reperfusion model was established in rats and the histo-pathological changes were observed by Nissl staining. Apoptotic cells were detected with TUNEL and the expressions of the associated proteins, such as Bcl-2, cyto C and caspase-3 were determined with immunohistochemistry and Western blot. Results： The expressions of Caspase-3 and cyto C following LIR were significantly increased compared with those in the control group （P〈 0. 05） and peaked 24 h later. The expression of Bcl-2 was negatively associated with reperfusion time, The expressions of Caspase-3 and Bcl-2 gene were obvious in the hippocampus and the midbrain red nucleus region, which was the sensitive area to ischemic injury. Conclusion： Our results indicate that LIR can lead to apoptotic brain injury. Bcl-2, cyto C and Caspase-3 may play a vital role in regulating the neural apoptosis following LIR in rats.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2005年第6期660-662,F0003,共4页
Chinese Journal of Anatomy
