摘要
目的研究过热对脂多糖(LPS)介导的大鼠急性肺损伤/急性呼吸窘迫综合征(ALI/ARDS)的影响.方法雄性SPF级Wistar大鼠随机分为常温生理盐水组(C组),高温生理盐水组(H组),常温脂多糖组(L组),高温脂多糖组(HL组).持续描计动物直肠温度(Tr),并检测动物应激0,40,80,120min时血浆TNF-α,IL-6含量,动脉血气,血浆肺湿质量/干质量比值(W/D)以及肺病理改变.结果①HL组Tr上升到(43.04±0.11)℃,与C组,L组之间存在显著性差异(P<0.01),与H组之间不存在显著性差异(P>0.05);②HL组动物血浆TNF-α峰值(80min)及IL-6表达水平显著升高(P<0.01);③HL组HCO_(3)^(-),PaCO_(2)于致伤40min时即出现显著下降(P<0.01),致伤120min时分别为(10.42±1.06)mmol/L,(2.82±0.81)kPa;④HL组动物W/D显著性升高(P<0.05);肺急性微血管损伤有病理学改变.结论过热应激能够加重LPS介导的大鼠ALI/ARDS.
AIM:To observe the effect of hyperthermia on lipopolysaccharide(LPS)-mediated ALI/ARDS in rats.METHODS:Male specific-pathogen free Wistar rats were randomly assigned to the following groups:saline-injected normothermic control(C-Group),saline-injected heat exposed(H-Group),LPS-injected normothermic control(L-Group)and LPS-injected heat exposed(HL-Group).Rectal temperature(Tr)was continually monitored.Plasma levels of TNF-αand IL-6,arterial blood gas,lung wet-to-dry weight ratio(W/D),and lung histological change were observed at 0,40,80 and 120 min.RESULTS:①The rats in HL-Group displayed significantly higher values of Tr(43.04±0.11)℃compared with C/L-Group(P〈0.01).There was no significant difference between HL-Group and H-Group(P〉0.05).②Significant elevations of the peak TNF-αlevel(at 80 min)and IL-6 expression were seen in HL-Group(P〈0.01).③The values of HCO_(3)^(-)and PaCO_(2)significantly decreased at 40 min in HL-Group(P〈0.01).At 120 min,the values of HCO_(3)^(-)and PaCO_(2)decreased to(10.42±1.06)mmol/L and(2.82±0.81)kPa respectively.④Significantly increased W/D ratio and many pathological features of acute microvascular lung injury were seen in HL-Group.CONCLUSION:Hyperthermia may aggravate ALI/ARDS in LPS-injected rats.
作者
林晓静
邹飞
李亚洁
王斌
韩雪琳
贺文静
LIN Xiao-Jing;ZOU Fei;LI Ya-Jie;WANG Bin;HAN Xue-Lin;HE Wen-Jing(Department of Nursing,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Institute of Public Hygiene and Tropic Medicine,Southern Medical University,Guangzhou 510515,China)
出处
《第四军医大学学报》
北大核心
2005年第24期2255-2257,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目(C030315)
军队"十五"医药卫生科研基金项目(01MA133)
