摘要
目的比较酶消化法、组织贴块法所培养大鼠肺动脉平滑肌细胞(pulmonaryarterialsmoothmusclecell,PASMC)的生长曲线、蛋白质含量、胞内[Ca2+]含量上的差异性,探讨不同方法是否可以用于同一系列的信号通路研究。方法酶消化法、组织贴块法分别培养PASMC,经过纯化以及免疫组化鉴定后,采用改良Lowry法测定蛋白质含量,采用荧光指示剂法测定胞内[Ca2+]含量,光镜下观察生长曲线。结果两种方法均顺利建立了纯PASMC系,但是在生长曲线和蛋白质含量上存在一定程度的差异,在胞内[Ca2+]含量上差异有显著性意义(P<0.05)。结论在同一系列的信号通路研究中应该尽量采用其中一种细胞培养方法。
Objective To compare the growth curve, protein content, calcium ion content of pulmonary arterial smooth muscle cells （PASMCs） cultured by enzyme digestion and tissue-sticking methods. To investigate whether PASMCs cultured by two ways could be offered to one series of signaling pathway studies. Methods The protein content was determined by upgraded-lowry, the calcium ion content by fluorescence indicator. The growth of PASMCs was observed by light microscope. Results There existed certain difference in protein content, growth curve between PASMCs cultured by the two methods, especially in calcium ion content （P 〈0. 05）. Conclusion Pure PASMCs cultured only by one way should be used in a series of signaling pathway studies.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2005年第24期2425-2427,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目(30400200)~~
关键词
肺动脉平滑肌细胞
细胞培养
蛋白质含量
pulmonary arterial smooth muscle cell
cell culture
protein content
