摘要
本研究用10种限制性内切酶对蜂猴和树鼩肝脏线粒体DNA进行了分析。蜂猴线粒体DNA,BglⅠHindⅢ、PstⅠ、SalⅠ和XhoⅠ均各只1个切点、EcoRⅠ、BglⅡ和PvuⅡ各有2切点,BamHⅠ和EcoR Ⅴ分别有3个切点。对于树鼩线粒体DNA,BglⅡ、HindⅢ、PstⅠ、SalⅠ和XhoⅠ均只1个切点,BamHⅠ BglⅠ和PvuⅡ分别有2个切点,EcoRⅠ有3个切点,EcoR Ⅴ有4个切点。根据单酶和双酶解片段的数目和分子量,建立了蜂猴和树鼩线粒体DNA的物理图谱。
The mitochondrial DNAs (mtDNAs) from slow loris (Nycticebus caucang bengalensis) and tree shrew (Tupaia belangeri chinensis) liver were digested with ten restriction endonucleases. One site for Bgl Ⅰ , Hind Ⅲ, Pst Ⅰ , Sal Ⅰ , Xho Ⅰ , two sites for EcoR Ⅰ , Bgl Ⅱ , Pvu Ⅱ ; and three sites for BamH Ⅰ , EcoR Ⅴ were found on the mtDNA from slow loris. One site for Bgl Ⅱ, Hind Ⅲ, Pst Ⅰ , Sal Ⅰ , Xho Ⅰ ; two sites for BamH Ⅰ , Bgl Ⅰ , Pvu Ⅱ ; three sites for EcoR Ⅰ ; and four sites for EcoR Ⅴ were found on the mtDNA from tree shrew. The mtDNAs were also cleaved by double-enzyme digestions with the above mentioned enzymes. The fragments thus obtained and their molecular weights were estimated by agarose gel electrophoresis. Restriction maps for slow loris and tree shrew were made according to the number and the size of those fragments obtained by single and double digestions. The distribution of cleavage sites were found not to be random.
关键词
线粒体
DNA
蜂猴
树QU
物理图谱
Mitochondrial DNA, Restriction map, Slow loris, Tree shrew
