摘要
提出了一种提取分离玉米浸泡水中蛋白质及脂多糖的方法。首先将大部分蛋白质分离出来,然后利用脂多糖易被活性炭吸附的特点,用活性炭将脂多糖从经过浓缩和去除了蛋白质的浸泡水中吸附分离出来。再使脂多糖从活性炭上脱附,同时考虑脂多糖的提纯。本文将脂多糖脱附与提纯两步合并为一步,通过活性炭色谱分离实现。得到脂多糖初步产品后,为了提高其经济价值,采用色谱分离对脂多糖进行提纯,最终使产品纯度达到97.4%。
In this article, we have introduced a new method for extracting protein and lipopolysaccharide in the soak water from maize starch factory. The protein is contained in the most part of the solid residue in the soak liquid. Though the lipopolysaccharide is the material of the highest value in the water, it is so little quantitatively and sparsely distributed. As a result, it is not available to have the lipopolysaccharide separated for the first step. Therefore, we have studied some of the important methods to separate protein from the solution and put forward a new craftwork so as to use the three means jointly to separate them, The first means is named ＂equal-charge point＂ method. The second method is to separate most of the protein and amylose and other solid residue with high molecular weight by super-filteration. And the third is to use chloride acetic acid to separate protein and other solid residue from it. Our final separating rate of protein is 94.4 percent. Thus, we have succeeded in achieving our inclination. Since the active carbon can easily adsorb lipopolysacchafide. It is rather easy to separate lipopolysaccharide from the liquid by active carbon. Of course the active carbon will also absorb some of the rudimental protein and amylose. The next step is to take out the lipopolysaccharide from active carbon and purify it. This step can be done by using a method called chromatogram separation. After extracting lipopolysaccharide with active carbon, it can be taken off from the active carbon by 10 mmol/L Tris-HCl/10 mmol/L NaCl. To further increase its economic value, we have also decided to purify it by a method called chromatogram separating. The lipopolysaccharide content of our ultimate product is 97.4% .
出处
《安全与环境学报》
CAS
CSCD
2005年第6期23-25,共3页
Journal of Safety and Environment
关键词
农产品贮藏与加工
玉米浸泡水
回收
活性炭
蛋白质
脂多糖
store and process of agricultural products
maize soak
recycle
active carbon
protein
lipopolysaccharide
