摘要
麦角菌［Ｃｌａｖｉｃｅｐｓｐｕｒｐｕｒｅａ（Ｆｒ．）Ｔｕｌ．ＡＴＣＣ２００１９］发酵生产麦角隐亭的最佳碳源是蔗糖或工业葡萄糖，蔗糖的最适浓度为３０％，麦角隐亭产量为９７．８—１０９．３μｇ／ｍＬ；发酵前期在１０％蔗糖培养基中再补加２０％蔗糖，可使产碱量比直接用３０％蔗糖的产碱量增加到１１１．９μｇ／ｍＬ．最佳氮源是酵母粉、蛋白胨、蚕蛹粉和黄豆饼粉．正交Ｌ１６（４２×２９）证明，硫酸镁是１０种发酵条件中对该菌株产麦角隐亭的量影响最大的一种．
Ergocryptine is produced by Claviceps purpurea (Fr.) Tul. ATCC 20019 in submerged liquid culture under aerobic condition. Sucrose and industrial glucose are the suitable carbon sources when citric acid is present. The suitable sucrose concentration is 30% and the amounts of alkaloid are 97.8-109.3μg/mL. Adding 20% sucrose again to a liquid culture containing 10% sucrose at the earlier fermentation period can enhance alkaloid production. The most favourable nitrogen sources selected are yeast extract, peptone, silkworm chrysalis and soybean cake powder. It is demonstrated that magnesium sulphate is the most important effective factor for alkaloid production by latin square L16(4 2×2 9).
出处
《南京师大学报（自然科学版）》
CAS
CSCD
1996年第3期63-68,共6页
Journal of Nanjing Normal University(Natural Science Edition)
