摘要
在C18柱上以V(甲醇):V(水)—25:75的溶液为流动相,流速1.0m l/m in,检测波长205nm,柱温25℃,同时分离测定市售感冒液中扑尔敏、扑热息痛的含量,扑尔敏、扑热息痛的检出限为1.26m g/L、0.02m g/L,线性范围分别为0.1m g/L^0.3m g/L、10 m g/L^30 m g/L。方法用于市售感冒药样品的分析,平均回收率扑尔敏为98.3%(RSD=1.58%n=5),扑热息痛为98.9%(RSD=1.92%n=5),方法简便、快速结果准确,结果较为满意。
Objective： To developed a new method for the determination of coldrex （ Chlorpheniramine and Paracetamol ）. Method： The determination was carried out by using C18 chemical bonded silicagle as a solid, methanol-water （25：75） as a mobile phase,the flow rate of 1.0mL/min, the injection volume of 10μl, the UV detection wavelength of 210nm and the column temperature of 25℃. Results： The concentration range of chlorpheniramine and paracetamol from 0. 1mg/L-0. 3mg/L and 10 mg/L - 30 mg/L,respectively with their detection limits of 1.26mg/ L and 0. 02mg/L, respectively . Conclusion： The method has been used to determine Chlorpheniramine and Paracetamol in sample and the average recoveries 98.3% and 98.9%. The proposed method is simple ,rapid,and accurate.
出处
《黑龙江医药科学》
2005年第6期34-35,共2页
Heilongjiang Medicine and Pharmacy
基金
黑龙江省普通高等学校骨干教师创新能力资助计划项目(1054G055)
关键词
高效液相色谱法
扑尔敏
扑热息痛
感冒药
high performance liquid chromatography
Chlorpheniramine
Paracetamol
coldrex
