摘要
以金山57×金山630的杂交后代所得的F1分离群体为材料,按F1单株抗性分群,建立抗病池和感病池,分别以其为摸板进行RAPD分析,以获得差异片段。应用RAPD技术筛选了320个随机引物,其中仅1个引物即S213-500在抗感池间扩增出了多态性谱带。应用结果表明该标记与田间鉴定的吻合率较高,能得到重复性验证和可靠的差异性扩增片段,可以作为抗I型薯瘟基因的连锁标记。
Bulked DNA from Ft segregating population of the cross Jinshan 57 x Jinshan 630 was used for RAPD analysis. 320 primers were used for the election of differential fragment, only one of which had differential amplification fragment from resistant and susceptible population. RAPD analysis of the resistant germplasm and individuals of Ft segregating population with one prime（ S213 -500） identified the truthfulness and relia- bility of the polymorphic amplification product. It suggested that this amplified product was linked with the Bacterial wilt resistant gene,and could be used as linked marker of the Bacterial wilt resistant gene.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2005年第6期861-863,938,共4页
Acta Agriculturae Universitatis Jiangxiensis
基金
福建省自然科学基金(B0110017)
国家863项目(2003AA207140)
关键词
甘薯
薯瘟病
分子标记
sweet potato
Bacterial wilt
molecular marker
