摘要
目的:制定骨痛宁搽剂(淫羊藿,马钱子,麻黄等)的质量标准.方法:采用薄层色谱法对处方中麻黄、三七、桂枝、丁香、洋金花等进行定性鉴别;采用高效液相色谱法对方中淫羊藿中淫羊藿苷、马钱子中士的宁进行含量测定.结果:TLC分离效果好,斑点清晰;淫羊藿苷在0.2～1.2 μg范围内线性关系良好,r=0.999 8,平均回收率100.3%,RSD=1.5%,士的宁在0.4～4.8μg范围内线性关系良好,r=0.999 7,平均回收率为99.17%,RSD=1.4%.结论:该方法准确可靠地进行定性、定量检测,能有效地控制制剂的质量.
AIM ： To establish the method for quality control of Gutongning Liniment （ Herba Epimedii, Semen Strychni, Herba Ephedrae, etc. ） METHODS： Herba Ephedrae, Radix Notoginseng, Ramulus Cinnamomi, Flos Caryophylli, Flos Daturae were identified by TLC, icariin content in Herba Epimedii and strychnine content in Se- men Strychni were determined by HPLC. RESULTS ： The identified characteristics of chromatography were distinct and the spots were clear. Icariin showed a good linear relationship at a range of 0.2-1.2μg, r=0.999 8. The average recovery was 10.3%, and RSD was 1.5 %. Strychnine showed a good linear relationship at a range of 0.8-4.8μg, r=0.999 7. The average recovery was 99.17%, and RSD was 1.4%. CONCLUSION： The methods are accurate and reliable, and can be used to control the quality of this preparation effectively.
出处
《中成药》
CAS
CSCD
北大核心
2005年第12期1400-1404,共5页
Chinese Traditional Patent Medicine
基金
科技部地方重大科技攻关项目(项目编号:00-D085)
